Biophotonics: Novel Lanthanide Bioassays

该提案概述了一个基于弱荧光或发光镧系螯合物共振增强的功能化分子探针的项目。镧系螯合物有几个吸引人的特性，作为荧光指示器使用，但遭受低吸收截面和量子效率。镧系螯合物的亮度可以在金属颗粒或充当天线的岛的存在下增强。由于这种增强随距离的变化而变化，因此增强可以用作报告局部生物活性的功能化或激活策略。本提案将重点关注低量子效率镧系螯合物，这些螯合物很少受到关注，但具有最大的相对增强效应，因此作为报告剂具有最大的动态范围。

Faris0523077This proposal outlines a project to develop functionalized molecular probes based on resonance enhancement of weakly fluorescent or luminescent lanthanide chelates. Lanthanide chelates have several attractive properties for use as fluorescent indicators, but suffer from low absorption cross-sections and quantum efficiency. The brightness of lanthanide chelates can be enhanced in the presence of metal particles or islands that act as antennas. Since this enhancement varies with proximity, the enhancement can be use as a functionalizing, or activation strategy for reporting local biological activity. This proposal will focus on low quantum efficiency lanthanide chelates which have received little attention but stand to have the largest relative enhancement effect, and therefore the greatest dynamic range as a reporting agent.