SST: Collaborative Research: Integrated Optical and Electrical Single Molecule Sensors

0528714 Hawkins光学和电学传感技术使人们能够研究气相或液相中的单个分子，从而在非常基础的层面上理解物理，化学和生物过程。提出了一种集成传感器平台，其在传感器阵列配置中提供单分子分辨率、极小的气体或液体样品体积、平面架构和高通量。这种传感器对于提供快速、可靠和鲁棒的分析工具是非常期望的。不需要庞大的三维设置，这些传感器也可以在实验室外使用，并可能影响各种领域，从基因组学和蛋白质组学毒理学和污染监测在空气和water.Intellectual优点：所提出的方法是基于结合纳米孔技术与单分子电分辨率和反共振反射光学（ARROW）波导与液体核心。本研究的主要目的如下：1.建立一种新型的集成光学传感器，同时提供单分子荧光灵敏度，并允许并行传感器阵列架构。2.将纳米孔作为“智能门”与光波导集成。纳米孔将控制分子进入光学检测通道，同时提供单分子分辨率的电学传感能力.利用光电感测的新组合研究基因组学及蛋白质组学中的生物分子，以增进对单分子水平的分子生物学的了解。虽然这个概念是，在原则上，同样适用于在气相中的分子的传感，本提案的重点是对液体的生物分子的研究在单分子水平上的集成传感器。更广泛的影响：上述研究计划具有显着的更广泛的影响。它将推进光学传感器科学和技术，并提供集成光学，分子生物学和半导体器件制造领域的研究生培训。本科研究将被纳入该计划。在UCSC，学生将参加现有的UC LEADS（代表性不足的少数民族）或COSMOS（K-12学生）计划下的项目。在杨百翰大学，本科生以及来自代表性不足的群体（杨百翰大学SOAR计划）的高中生将在夏季参与项目的制造方面。

0528714HawkinsOptical and electrical sensing has enabled the study of individual molecules in the gas or liquid phase, leading to an understanding of physical, chemical and biological processes on a very fundamental level. An integrated sensor platform is proposed that provides single-molecule resolution, extremely small gas or liquid sample volumes, planar architecture, and high throughput in a sensor array configuration. Such a sensor is highly desirable for providing fast, reliable and robust analytical tools. Without the need for bulky three-dimensional setup, these sensors can also be used outside a laboratory and could impact a variety of fields ranging from genomics and proteomics to toxicology and pollution monitoring in both air and water.Intellectual merit: The proposed approach is based on combining nanopore technology with single-molecule electrical resolution and antiresonant reflecting optical (ARROW) waveguides with liquid cores. The research has the following primary goals: 1. To build a new type of integrated optical sensor that simultaneously provides single-molecule fluorescence sensitivity and allows for parallel sensor array architectures. 2. To integrate nanopores as "smart gates" with the optical waveguides. The nanopores will control molecule access in the optical detection channel and simultaneously provide electrical sensing capability with single molecule resolution.3. To use the novel combination of optical and electrical sensing to study biomolecules in genomics and proteomics for enhancing understanding of molecular biology on the single molecule level. While the concept is, in principle, equally applicable to sensing of molecules in the gas phase, the focus of this proposal is on integrated sensors of liquids for biomolecular studies on the single molecule level.Broader Impact: The research program outlined above has significant broader impact. It will advance optical sensor science and technology and provide graduate student training in the fields of integrated optics, molecular biology, and semiconductor device fabrication. Undergraduate research will be integrated into the program. At UCSC, students will participate in the project under the existing UC LEADS (underrepresented minorities) or COSMOS (K-12 students) programs. At BYU, undergraduates as well as high school students from underrepresented groups (BYU SOAR program) will be involved in the fabrication aspects of the projects during the summer.