Molecular investigation of vascular lumen formation in the developing mouse aorta

血管是具有中央血管腔的多细胞管，为组织提供氧气、营养物质以及发育信号(Lammert等人，2001年)。尽管它们对胚胎发育和成年生活很重要，但血管内皮细胞聚集形成管状血管的分子机制仍然不完整，从而允许血液流入和流出每个组织。我们最近的数据表明，在发育中的小鼠胚胎中，主动脉内皮细胞形成多细胞索，其中内皮细胞部分死亡并相互分离，在它们之间形成血管腔(Strilic等人，2009年)。在这里，我们想要研究CD34-sialomucins的抗黏附作用，以及血管内皮生长因子和Eph受体介导的内皮细胞分离在所有哺乳动物和人类最大的动脉血管--主动脉形成过程中的作用。

Blood vessels are multicellular tubes with a central vascular lumen that provides tissues with oxygen, nutrients as well as developmental signals (Lammert et al., 2001). Despite their importance for embryonic development and adult life, the molecular mechanism remains fragmentary by which vascular endothelial cells assemble to form lumenized vascular tubes, thus allowing blood flow to and from each tissue. Our recent data demonstrate that, in developing mouse embryos, aortic endothelial cells form multicellular cords, in which the endothelial cells partially deadhere and separate from each other to form a vascular lumen between them (Strilic et al., 2009). Here we want to study the role of anti-adhesive CD34-sialomucins as well as the role of VEGF and Eph receptor mediated endothelial cell separation during formation of the aorta, the largest arterial blood vessel in all mammals and humans.