Single-molecule fluorescence spectroscopy and imaging of the structure and dynamics of macromolecular assemblies (A06)

该项目的核心目标是开发一种荧光光学方法，用于单荧光分子的三维定位和共定位，具有~ 1nm的各向同性分辨率。为此，我们将结合两种技术：基于单分子定位的超分辨率显微镜（STORM， PALM， PAINT, MinFlux）和金属诱导能量转移（MIET）成像。我们将使用这种方法对荧光标记的大分子复合物的结构细节进行成像，具有纳米分辨率和高特异性，而且还可以解决结构变化的时间动态，时间分辨率低至几纳秒。

The core goal of the project is the development of a fluorescence-optical method for three-dimensional localization and co-localization of single fluorescent molecules with ~1 nm isotropic resolution. For this purpose, we will combine two techniques: single-molecule localization based super-resolution microscopy (STORM, PALM, PAINT, MinFlux) with metal Induced Energy Transfer (MIET) imaging. We will use this method to image structural details of fluorescently labelled macromolecular complexes with nanometer resolution and high specificity, but also to resolve temporal dynamics of structural changes with a temporal resolution down to a few nanoseconds.