Protein cross-linking of macromolecular complexes (A10)

我们将进行蛋白质交联结合质谱（MS）休息和刺激的B细胞。关键信号分子的交联相互作用将与B细胞在B细胞受体（BCR）刺激下的磷酸化状态有关，并将获得磷酸化如何影响分子相互作用的信息。此外，我们将利用紫外光和化学交联剂建立蛋白质- dna交联结合体外重组蛋白质- dna复合物以及体内和离体系统的质谱。将获得dna -蛋白质系统（如染色质）中的结构功能关系。

We will perform protein cross-linking combined with mass spectrometry (MS) of resting and stimulated B cells. The cross-linked interactome of key signaling molecules will be related to the phosphorylation states of the B cells upon B cell receptor (BCR) stimulation and information on how phosphorylation influences molecular interactions will be obtained. Further, we will establish protein-DNA cross-linking combined with MS of in vitro reconstituted protein-DNA complexes and in in vivo and ex vivo systems using UV-light and chemical cross-linker. Structural function relationships in DNA–protein systems like chromatin will be obtained.