The Maize TILLING Project: Reverse genetics of maize point mutations

PI：克利夫·韦尔，普渡大学单碱基改变是功能基因组学中有价值的工具，因为它们可以揭示基因产物活性、相互作用和特异性的变化。靶向诱导有限损伤基因组(TILLING)是一种可以在大量诱变生物中筛选出任何感兴趣基因有单碱基变化的个体的技术。玉米分耕计划(MTP)提供了一项服务，允许玉米研究人员识别和获得任何基因缺陷的突变品种的种子。这项奖励的目的是使MTP在财政上能够自给自足。此外，MTP正在创建和评估近交系B73和W22的新诱变品系，为社区提供最佳诱变种子的通用资源。这些突变株系将通过一个强调服务不足的社区以及中学生和教师的外联计划进行详细的表型鉴定。获取项目成果：将公开所有表型和突变数据(http://genome.purdue.edu/maizetilling).在耕作客户收到他/她的订单六个月后，序列数据将被发布在弗雷德·哈钦森癌症研究中心(http://fhcrc.org:9355/search.html)和MaizeGDB(http://www.plantgdb.org/ZmGDB/).))的主耕作服务器上库存将通过玉米遗传合作库存中心(http://w3.ag.uiuc.edu/maize-coop/).)提供

PI: Cliff Weil, Purdue University Single base changes are valuable tools in functional genomics because they can reveal changes in gene product activity, interaction and specificity. Targeting Induced Limited Lesions IN Genomes (TILLING) is a technology that can screen large populations of mutagenized organisms for those individuals that have single base changes in any gene of interest. The Maize TILLING Project (MTP) provides a service allowing maize researchers to identify and obtain seed for mutant varieties with defects in any gene. This award is designed to allow the MTP to become financially self-supporting. In addition, MTP is creating and evaluating new mutagenized lines of the inbreds B73 and W22 to provide the community with a general resource of optimally mutagenized seed. These mutant lines will be phenotyped in detail through an outreach program emphasizing underserved communities and secondary school students and teachers. Access to project outcomes: All phenotypic and mutation data will be made publicly available (http://genome.purdue.edu/maizetilling). Six months after the TILLING client has received his/her order, sequence data will be posted at the main TILLING server at Fred Hutchinson Cancer Research Center (http://fhcrc.org:9355/search.html) and MaizeGDB (http://www.plantgdb.org/ZmGDB/). Stocks will be made available through the Maize Genetics Cooperation Stock Center (http://w3.ag.uiuc.edu/maize-coop/).