CBA: QEIB: Estimation and Interpretation of Allele Frequencies: Novel Theoretical Analyses and High-Throughput Quantitation by DNA Pooling

简单序列重复（SSRs）是在整个基因组中发现的重复DNA序列。在许多种类的生物体中，SSRs在不同的科学环境中进行了研究。相对于个体基因分型的标准方法，使用来自多个个体的SSR DNA的方案有可能提高实验室效率（在时间和金钱方面），用于许多种类的群体遗传研究。本研究探讨了利用混合条纹鲈鱼DNA估计SSR等位基因频率的实验室方法，以确定最佳方案。一组补充的数学分析将研究等位基因之间关联的估计，亚群体之间遗传结构的评估，遗传漂变的替代随机模型，以及新优势基因在结构群体中的空间传播率。通过DNA池进行等位基因频率估计有可能促进广泛的遗传调查，包括在疾病制图、法医学、病毒学和微生物生态学中的应用。此外，条纹鲈鱼在渔业和水产养殖业中具有重要的经济意义，数学分析将有助于有关条纹鲈鱼和其他种群具有保护和/或经济重要性的物种管理的种群遗传研究。最后，这项研究是跨学科的，涉及具有统计学和遗传学、生理学和生物技术、人口生态学和遗传学经验的研究人员，期望高级人员及其学生以及他们的科学将从互动中受益。

Simple sequence repeats (SSRs) are repetitive sequences of DNA that have been found throughout the genome. SSRs are studied in diverse scientific settings in many kinds of organisms. Relative to the standard approach of individual genotyping, protocols using SSR DNA pooled from multiple individuals have the potential to improve laboratory efficiency (in terms of time and money) for many kinds of population genetic studies. This study investigates laboratory methods for estimating SSR allele frequencies using pooled striped bass DNA to determine optimal protocols. A complementary set of mathematical analyses will investigate the estimation of associations among alleles, the assessment of genetic structure among subpopulations, alternative stochastic models for genetic drift, and the spatial rate of spread of novel, advantageous genes into structured populations.Allele frequency estimation by DNA pooling has the potential to facilitate a wide variety of genetic investigations, including applications in disease mapping, forensic science, virology, and microbial ecology. Moreover, striped bass is economically important in the fishing and aquaculture industries, and the mathematical analyses will facilitate population genetic studies concerning the management of this and other species whose populations are of conservation and/or economic importance. Finally, this research is interdisciplinary, involving investigators with experience in statistics and genetics, physiology and biotechnology, and population ecology and genetics, and the expectation is that the senior personnel and their students, as well as their science, will benefit from the interaction.