Functionalization of Polymer Brushes for Selective Capture and Analysis of Phosphorylated and Methionine-Containing Peptides

Bruening/Michigan State在分析和表面化学计划的支持下，Michigan州立大学的Merlin Bruening教授小组正在开发选择性捕获蛋白质和肽的方法，用于基质辅助激光解吸/电离质谱分析。 生物分子直接捕获在用于样品引入的板上，避免了耗时的分离技术和相关的样品损失。 具体而言，控制样品探针上的聚合物刷的生长和这些刷与Fe（III）络合物的衍生化将被测试，以使选择性结合和高灵敏度的分析磷酸化肽。 还将开发在样品板上选择性捕获含甲硫氨酸肽的方法。 控制样品板上聚合物刷的密度、厚度、成分和膨胀对于在这两个区域实现低检测限至关重要。 蛋白质磷酸化的鉴定和定量对于理解细胞调节和癌症等疾病的过程至关重要。 含甲硫氨酸的肽的选择性结合将减少来自消化混合物的信号的数量，但仍提供有效的蛋白质覆盖。 这有望大大简化复杂生物样品的分析。 该项目的工作将由包括高中，本科和研究生在内的各级学生进行，为重要的生物化学和分析研究领域提供广泛的视角。

ABSTRACT CHE-0616795Bruening/Michigan StateWith the support of the Analytical and Surface Chemistry Program, the group of Professor Merlin Bruening at Michigan State University is developing methods for selectively capturing proteins and peptides for analysis by Matrix-Assisted Laser Desorption/Ionization mass spectrometry. The biomolecules are captured directly on the plate used for sample introduction, avoiding time-consuming separation techniques and associated sample loss. Specifically, controlled growth of polymer brushes on the sample probe and derivatization of these brushes with Fe(III) complexes will be tested for enabling selective binding and highly sensitive analysis of phosphorylated peptides. Methods for selective capture of methionine-containing peptides on sample plates will also be developed. Control over the density, thickness, composition, and swelling of polymer brushes on sample plates will be vital for achieving low detection limits in both areas. Identification and quantitation of protein phosphorylation is vital for understanding processes such as cell regulation and diseases such as cancer. Selective binding of the methionine-containing peptides will decrease the number of signals from a digest mixture but still provide effective protein coverage. This is expected to greatly simplify the analysis of complex biological samples. Work on this project will be performed by students at all levels including high school, undergraduate, and graduate students, providing a broad perspective on important biochemical and analytical research areas.