Acquisition of Circular Dichroism Spectropolarimeter

这个奖项是为了获得一个圆形二色（CD）分光偏振计与停止流动和全荧光附件。该仪器将用于测量DNA低聚物和蛋白质由于限制效应的变性转变和构象展开。研究人员将使用CD仪器作为差示扫描量热法和等温滴定量热法的补充，用于以下用途：监测DNA双链形成时的构象顺序，确定该参数是否依赖于序列，测量三核苷酸DNA双链的变性过程，以及测量药物与DNA结合时的构象变化。获得这台CD仪器将极大地加强学院普通化学、生物化学、无机化学和物理化学课程的探究性课程，以及对本科生研究人员的好处。获得这台仪器也加强了这所本科学院和阿贡国家实验室之间的合作。

This award is for the acquisition of a circular dichroism (CD) spectropolarimeter with stopped flow and total fluorescence accessories. This instrument will be used to measure the denaturation transition and conformational unfolding of DNA oligomers and proteins due to confinement effects. The investigators will use the CD instrumentation to complement Differential Scanning Calorimetry and Isothermal Titration Calorimetry for the following uses: to monitor conformational ordering upon DNA duplex formation, to determine whether this parameter is sequence dependent, to measure denaturation process of trinucleotide DNA duplex, and to measure conformational change upon drugs binding to DNA. Acquisition of this CD instrumentation will greatly enhance inquiry-based curriculum in the general chemistry, biochemistry, inorganic chemistry and physical chemistry courses at the college, in addition to its benefits for undergraduate student researchers. Acquisition of this instrumentation also strengthens the collaboration between this undergraduate college and Argonne National Laboratory.