SGER: The Development of an in PlantaTransformation Technology to Facilitate Functional Genomics in Wheat

Pi：Janice M.Zale(田纳西大学)Copi：Kateen M.Devos(佐治亚大学)目前的小麦转化技术技术困难、耗时、劳动密集、成本高，而且依赖于组织培养阶段。此外，还没有转化技术来促进对小规模或大规模功能基因组学候选基因的测试。因此，迫切需要开发一种高通量转化技术，特别是在小麦等顽固作物物种中。该项目的目标是解决这一需求，并开发一种高效的植物转化协议，用于小麦，类似于用于模式植物拟南芥的转化协议。具体目标是：(1)建立和优化小麦品系藏红花T-DNA标签小麦转化体系中的标准体系，并生成T-DNA标签小麦转化体；(2)测序和定位12个独立的藏红花转化体的T-DNA插入位点，以验证转化子是真实的、稳定的，并作为生产T-DNA标签品系的第一步。广泛影响：这种体系的开发将对小麦功能基因组学产生巨大影响，并可能在其他谷物中产生影响。此外，该项目将为本科生和研究生提供培训，并通过论文、研讨会和会议报告广泛传播研究成果。

PI: Janice M. Zale (University of Tennessee)CoPI: Katrien M. Devos (University of Georgia)Current wheat transformation technologies are technically difficult, time-consuming, labor intensive, expensive, and rely upon a tissue culture phase. Furthermore, there are no transformation technologies that facilitate testing of candidate genes for small or large scale functional genomics. Thus, there is an urgent need for the development of a high throughput transformation technology, particularly in recalcitrant crop species such as wheat. The goal of this project is to address this need and develop an efficient in planta transformation protocol for use in wheat similar to that used for the model plant Arabidopsis thaliana. The specific objectives are to (1) develop and optimize a standard in planta protocol using the wheat line Crocus and generate T-DNA tagged wheat transformants and (2) sequence and map the T-DNA insertion sites of 12 independent Crocus transformants to validate that the transformants are genuine, stable, and as a first step in producing T-DNA tagged lines.Broader Impacts: The development of such a protocol would have a tremendous impact for functional genomics in wheat and potentially in other cereals. In addition, the project will provide for training of undergraduate and graduate students and for the broad dissemination of research results in papers, seminars, and conference presentations.