Role of FHY3 and FAR1 in transcriptional regulation of phytochrome A signaling in Arabidopsis

项目摘要 主要研究者：Haiyang WangCo-PI：Matthew Hudson提案编号：IOS-0641639提案标题：FHY 3和FAR 1在拟南芥光敏色素A信号传导的转录调节中的作用植物使用一系列光感受器来感知周围的光环境并进行适应性生长和发育。光敏色素A（phyA）是高等植物中介导各种远红光诱导反应的初级光感受器。现在广泛接受的是，phyA的光激活启动信号转导级联，导致调节基因表达的变化和随后的光形态发生发育。FHY 3和FAR 1是拟南芥中两个重要的phyA信号正调控因子，它们与Muxylase类转座酶具有重要的氨基酸相似性。然而，它们的分子功能以及它们如何适应phyA信号通路仍然是难以捉摸的。该项目的中心目标是证实FHY 3和FAR 1可能定义了一类新的转录调节因子的假设，可能是从古老的突变体样转座因子驯化的，其在细胞核中起作用以调节phyA响应基因的表达和远红光下的光形态发生发育。三个具体目标是：1。研究FHY 3和FAR 1在植物细胞中的转录调控活性。鉴定其直接靶基因和功能特征; 3.探讨它们与光感受器phyA的功能关系。这些研究的结果将导致更好地了解FHY 3和FAR 1在调节phyA介导的基因表达，并为建立连贯的phyA信号通路提供关键信息。预计这项工作将产生更广泛的影响，包括培训不同层次的科学家，从本科生到研究生和博士后研究员。PI还将参与当地高中教师的培训，并在当地小学课堂上介绍由印第安纳州大学的Roger P. Hangarter教授创作的“植物运动”电影，向学生们教授植物对光线和其他环境信号的反应。

Project Abstract PI: Haiyang WangCo-PI: Matthew HudsonProposal Number: IOS-0641639Proposal Title: Role of FHY3 and FAR1 in transcriptional regulation of phytochrome A signaling in ArabidopsisPlants use an array of photoreceptors to sense the ambient light environment and undergo adaptive growth and development. Phytochrome A (phyA) is the primary photoreceptor for mediating various far-red light induced responses in higher plants. It is widely accepted now that photo-activation of phyA initiates a signal transduction cascade that leads to regulated gene expression change and subsequent photomorphogenic development. FHY3 and FAR1, two homologous proteins that share significant amino acid similarities with the MuDRA class of transposases, define two essential positive regulators of phyA signaling in Arabidopsis. However, their molecular function and how they fit in the phyA signaling pathway have remained elusive. The central goal of this project is substantiate the hypothesis that FHY3 and FAR1 may define a novel class of transcriptional regulators, possibly domesticated from an ancient Mutator-like transposable element, that act in the nucleus to regulate phyA-responsive gene expression and photomorphogenic development under far-red light. The three specific aims are: 1. To characterize the transcriptional regulatory activity of FHY3 and FAR1 in plant cells; 2. To identify their direct target genes and functional characterization; and 3. To investigate their functional relationship with the photoreceptor phyA. Results from these studies will lead to a better understanding of FHY3 and FAR1 in regulating phyA-mediated gene expression and add critical information toward establishing a coherent phyA signaling pathway. The broader impacts expected from this work include training of different levels of scientists, ranging from undergraduate students, to graduate students and postdoc fellows. The PI will also participate in the training of local high school teachers and introduce the "Plants-In-Motion" movies, which were created by Professor Roger P. Hangarter at Indiana University, in local elementary school classes to teach school children about plant responses to light and other environmental signals.