Simple Methods for Oligonucleotide Purification

本项目的重点是开发适合大规模纯化寡核苷酸的方法。有两个特定的目标：(1)开发一种使用可聚合旋盖剂来盖住失效序列的寡核苷酸纯化方法；(2)开发一种使用可聚合的酰胺磷来使全长序列的5'端功能化的寡核苷酸纯化方法。第一种方法用于纯化大量5- 20米的寡核苷酸，而第二种方法用于纯化20- 100米的寡核苷酸。通过这个奖项，有机和大分子化学项目支持了密歇根理工大学化学系方世岳教授的研究。方教授的研究工作围绕着大规模纯化寡核苷酸的简单方法的发展。方教授项目的成功完成可以使寡核苷酸易于获得，从而有助于利用寡核苷酸开发实用的治疗剂。

This project is focused on the development of methods suitable for the large scale purification of oligonucleotides. There are two specific objectives: (1) to develop an oligonucleotide purification method using a polymerizable capping agent to cap failure sequences and (2) to develop an oligonucleotide purification method that uses a polymerizable phosphoramidite to functionalize the 5'-end of full-length sequences. The first approach is designed for the purification of large quantities of 5- to 20-mers oligonucleotides while the second one is designed for the purification of 20- to 100-mers. With this award, the Organic and Macromolecular Chemistry Program is supporting the research of Professor Shiyue Fang, of the Department of Chemistry at Michigan Technological University. Professor Fang's research efforts revolve around the development of simple methods for the large scale purification of oligonucleotides. Successful accomplishment of Professor Fang's project could make oligonucleotides readily available, thus assisting the development of practical therapeutic agents using oligonucleotides.