Collaborative Research: NanoCytometry: A Point-of-Care Technology for Monitoring Chronic Leukemia Patients

CBET-0651799孙 该提案的目标是开发一种NanoCytometer（孔加微流体芯片和传感器），以按大小，类型和膜身份对细胞进行分类。这项工作将继续构建一个阵列NanoCytometer。长期的研究目标是提供一个快速、方便的白血病和其他慢性疾病的即时诊断系统。当小的血液样本通过表面涂有某些蛋白质的纳米孔时，细胞表面相互作用，流速和细胞大小可以导致细胞计数和识别。由于细胞通过孔而产生的电子信号、其幅度和时间间隔可用作细胞计数、大小和类型的指示。基于这一事件的物理学，研究人员将首先开发纳米多孔结构阵列，并证明他们的假设，最后将其与微流体和电子传感能力相结合。这项研究的更广泛影响包括三名女性研究人员之间的合作，每个人都是不同领域的专家。调查人员描述了鼓励年轻妇女和少数民族从事工程、科学和数学的活动。该小组对这些活动的领导将为参与者提供榜样。描述了一个暑期科学夏令营，让学生获得在拟议的NanoCytometer上进行研究的经验。更广泛的社会影响是嵌入在这项研究的目标-一个点的护理诊断设备的慢性疾病。

CBET-0651799 Sohn The goal of this proposal is to develop a NanoCytometer (a pore plus microfluidic chip and sensor) to sort cells by size, type and membrane identity. The work will continue to construct an array NanoCytometer. The long term research goal is provide a point of care diagnostic system for leukemia and other chronic diseases, rapidly and conveniently. When small blood samples pass through nano-sized pores whose surfaces are coated with certain proteins, cell-surface interactions, the rate of flow and size of cells can lead to counting of cells and their recognition. The electronic signal due to the passage of cells thru the pore, its magnitude and time interval can used as indications of cell count, size and type. Based on this physics of this event, the investigators will first develop nano-porous structure arrays, and demonstrate their hypothesis, and finally integrate it with microfluidics and electronic sensing capabilities. The broader impact of this research includes the collaboration among three female investigators, each an expert in a different field. The investigators describe activities to encourage young women and minorities to engage in engineering, science and mathematics. Leadership of these activities by this team will provide role-model examples for the participants. A summer science camp is described for students to gain experience on research performed on the proposed NanoCytometer. Broader societal impact is embedded in the goal of this research--- a point of care diagnostic device for chronic diseases.