Electrohydrodynamic Instability Micromixing Within Two Phase Microfluidic Systems for Biological Purification Technology

项目编号：css -0553906项目负责人：Zahn， jeffrey合作单位：宾夕法尼亚州立大学项目名称：电流体动力学不稳定微混合在两相微流体系统中用于生物净化技术该项目建议研究有机-水两相微流，并创建一个能够执行液-液萃取技术的自主装置用于生物样品纯化。这项工作将通过实验、分析和计算方法扩展当前对微观几何多相流的理解和操作。两相流行为将研究使用荧光染料定位技术和微piv。通过电流体动力学（EHD）不稳定性来破坏流动，以增强两相的混合，从而增加可能发生液体萃取的表面积，并通过新型侧视微piv技术进行三维剖面重建。该方案将实验研究两相体系和EHD混合的流动行为，并将结果与分析和计算分析进行比较。本文还将扩展计算模型对EHD不稳定性的模拟。更广泛的影响这个问题是多学科的，汇集了工程和生物学的工具来解决一个问题，这对涉及芯片上样品制备的任何未来应用都是至关重要的。这项工作整合了跨学科技术，并研究了迄今为止很少或没有受到关注的DNA提取的强大方法。该技术将使样品制备高度集成的遗传筛选诊断。控制两相混合是一个关键的障碍，任何未来的应用，使用液体萃取分离程序的样品纯化之前分析。这项技术有望在医学基因检测、高通量DNA测序、生物战剂检测和水质监测等不同领域实现现场生物样本收集和分析。将特别强调在大学一级科学教育中向代表性不足的群体进行教育推广。这些活动将增强生物医学和纳米生物技术新兴领域的基础知识，并培训该领域的年轻科学家、工程师和教育工作者。

Proposal Number: CTS-0553906Principal Investigator: Zahn, JeffreyAffiliation: Pennsylvania State UniversityProposal Title: Electrohydrodynamic Instability Micromixing Within Two Phase Microfluidic Systems for Biological Purification TechnologyThis project proposes to study organic-aqueous two phase microflows, and create an autonomous device capable of performing liquid-liquid extraction techniques for biological sample purification.Intellectual MeritThe work will extend the current understanding and manipulation of multiphase flows in microgeometries through experimental, analytical and computational approaches. Two phase flow behavior will be studied using a fluorescent dye localization technique and microPIV. The flow is destabilized by an electrohydrodynamic (EHD) instability to enhance mixing of the two phases to increase the surface area over which liquid extraction may occur, monitored by a novel sideview microPIV technique for three dimensional profile reconstruction. The proposal will experimentally study the flow behavior of the two phase system and EHD mixing and will compare the results to analytical and computational analysis. The modeling of EHD instabilities by computational modeling will also be extended.Broader ImpactThe problem is multidisciplinary, bringing together tools from engineering and biology to attack a problem that is crucial for any future applications involving on-chip sample preparations. This work integrates interdisciplinary technologies and investigates a powerful approach to DNA extraction that has received little or no attention to date. This technique will enable sample preparation for highly integrated genetic screening diagnostics. Controlling two-phase mixing is a key hurdle for any future application that uses a liquid extraction separation procedure for sample purification prior to analysis. This technique holds major promise to enabling on-site biological sample collection and analysis for areas as disparate as medical genetic testing, high throughput DNA sequencing, biowarfare agent detection, and water quality monitoring. Special emphasis will be placed on educational outreach to under-represented groups in college level science education. These activities will enhance fundamental knowledge in the emerging field of BioMEMS and nanobiotechnology as well as train young scientists, engineers and educators in the area.