SBIR Phase II: Immunological Tools for Trimetasphere Fullerenes

这项小型企业创新研究（SBIR）二期研究开发了基于碳基纳米材料的抗体和免疫测定方法。这项研究将扩展第一阶段开发的免疫学工具，专注于抗富勒烯抗体的详细表征，并验证和选择经过验证的商业格式的免疫测定和试剂。商业形式将包括用于医疗和环境应用的酶联免疫吸附测定（ELISAs），减轻富勒烯/纳米管潜在毒性的中和方案和用于长期监测系统的生物传感器平台。以富勒烯抗体为亲和配体的生物传感器平台将包括石英晶体微天平和表面声波导。更广泛的影响将是为研究医学相关的纳米材料和监测制造过程中的纳米废物副产品提供全方位的免疫学工具。这些将是市场上的新产品，能够监测基于这些纳米材料的产品的使用，以确保其安全应用。

This Small Business Innovation Research (SBIR) Phase II research develops antibodies and immunoassays for studying therapeutics based on carbon-based nanomaterials. This research will expand the immunological tools developed in Phase I to focus on detailed characterization of anti-fullerene antibodies and validate and down-select immunoassays and reagents for validated commercial formats. Commercial formats will include enzyme-linked, immunosorbant assays (ELISAs) for medical and environmental applications, neutralization schemes for mitigating potential toxicity of fullerene/nanotubes and biosensors platform for long-term monitoring systems. Biosensor platforms based on fullerene antibodies as affinity ligands will include the quartz crystal microbalance and surface acoustic waveguide. The broader impacts will be to provide a full spectrum of immunological tools for studying the medically-related nanomaterials and monitoring nanowaste by-products during manufacturing processes. These will be new to the marketplace and enable monitoring of the use of products based on these nanomaterials to asssure their safe application.