Enhancing Molecularly Imprinted Polymer Binding Parameters via Reaction Analysis

PI：Mark E. Byrne机构：奥本大学提案编号：0730903标题：通过反应分析增强分子印迹聚合物结合参数这项工作将涉及用于识别模板分子的三维聚合物网络的形成和表征。近年来，大分子识别领域，分子印迹，开发了多孔的，高度交联的杂聚物作为鲁棒的识别矩阵。然而，在聚合反应分析领域内没有研究或进展，以研究网络形成及其对聚合物基质内的结合亲和力、选择性和结合位点数量的影响，这些是决定印迹有效性的主要参数。由于这些性质强烈依赖于网络结构，因此研究反应的细节非常重要。随着过渡应用和专业应用的不断发展，如功能性传感器薄膜、即时诊断、分析和药物递送载体，通过反应分析对聚合物结构进行表征和优化至关重要。聚合反应分析将导致更好地理解印迹机理，增强结合参数，并在印迹网络的应用潜力大幅增加。主要目标是：以乙基腺嘌呤-9-乙酸酯为模板分子，通过自由基光聚合合成印迹网络。网络组成的参数研究将随着引发剂类型、机理和浓度、模板/官能单体比率、交联单体类型和长度、温度和溶剂浓度的变化而发生;确定模板平衡结合参数，如亲和力、容量和选择性;分析模板聚合反应，以确定链增长和终止动力学，最终双键转化率和动力学链长。反应分析和相关的反应和结构参数将与模板的能力，亲和力和选择性;和。确定网络结构属性和动态模板传输。智力优势。推进和贡献的基本知识，形成的非线性聚合物结构，并发展模板分子如何影响大分子链的结构组织和动力学的理解。该提案中的结果首次通过反应分析和反应动力学的改变显示出增强的结合参数，假设这是由于结合位点的结构均一性增加以及稳定性和完整性增加。.在不同领域/学科的大量先进的知识，因为这项工作在分离科学，强大的化学/生物化学传感器涂层和功能的微米和纳米层，增强载体的持续释放的治疗剂，以及在精确的聚合策略的影响。更广泛的影响。采用聚合物科学技术来影响模板材料的合理设计和工程，这将影响大量的材料指令，推进创新技术和发现，并导致更好地整合技术中的印迹结构，以造福社会。.创建一个多层次，多元化，综合的研究团队，包括PI，2个代表性不足或少数民族博士研究生，和3个代表性不足或少数民族本科研究人员，重点是扩大代表性不足的群体参与研究的机会。.通过将聚合物科学，聚合物工程，材料工程，分子识别和化学工程原理整合到研究和教育项目中，为2名研究生和3名本科生提供多学科，协作研究培训和教育。.通过将聚合物工程的最新研究和发现与教育活动相结合，增加本科生对研究活动和职业的兴奋。.通过在各种媒体（聚合物科学和工程期刊，生物材料科学期刊，药物输送期刊）上发表结果以及向各种学会（材料研究学会，生物材料学会，美国化学工程师学会）发表会议报告来广泛传播结果。.将这项工作的变革性技术和研究纳入PI课程的课堂。.通过将这项工作的影响纳入奥本老虎夏令营的“聚合物展”，增加公众的理解，并激发K-12对科学和工程的兴趣。.为研究生和本科生提供指导，专业发展和职业指导，这将有助于建立一支准备充分的劳动力队伍。.通过研究生和本科生的研究指导经验促进教师发展。

PI: Mark E. Byrne Institution: Auburn University Proposal Number: 0730903TITLE: Enhancing Molecularly Imprinted Polymer Binding Parameters via Reaction Analysis This work will involve the formation and characterization of three-dimensional polymeric networks for the recognition of template molecules. In recent years, the field of macromolecular recognition, molecular imprinting, has exploited porous, highly-crosslinked heteropolymers as robust recognition matrices. However, there have been no studies or progress within the field in the area of polymerization reaction analysis to study the network formation and its influence on the binding affinity, selectivity, and number of binding sites within the polymer matrix, main parameters that determine the imprinting effectiveness. Since these properties are strongly dependent on the network structure, it is very important to study the details of the reaction. As transitional applications and specialized applications are pursued, such as functional sensor films, point-of-care diagnostics, assays, and drug delivery carriers, the characterization and optimization of the polymer structure via reaction analysis is paramount. Polymerization reaction analysis will lead to a greater understanding of the imprinting mechanism, enhancement of binding parameters, and a substantial increase in the application potential for imprinted networks. The major objectives are to: . Synthesize imprinted networks for the template ethyl-adenine-9-acetate via free-radical photopolymerization. A parametric study of network composition will occur with variations in initiator type, mechanism, and concentration, template/functional monomer ratio, crosslinking monomer type and length, temperature, and solvent concentration; . Determine template equilibrium binding parameters such as affinity, capacity, and selectivity; . Analyze template polymerization reactions to determine chain propagation and termination kinetics, final double bond conversion, and kinetic chain length. Reaction analysis and associated reaction and structural parameters will be correlated to template capacity, affinity, and selectivity; and . Determine network structural properties and dynamic template transport. INTELLECTUAL MERIT . Advance and contribute fundamental knowledge on the formation of recognitive polymeric structures and develop understanding of how template molecules affect the architectural organization and kinetics of macromolecular chains. Results within this proposal are the first to show enhanced binding parameters by reaction analysis and alteration of the reaction kinetics, hypothesized to be due an increased structural homogeneity and increased stability and integrity of binding sites. . Substantially advance knowledge across a diverse number of fields/disciplines since this work has impact in separation science, robust chemical/biochemical sensor coatings and functional micro- and nano-layers, enhanced carriers for the sustained release of therapeutic agents, as well as in precise polymerization strategies. BROADER IMPACTS . Employ polymer science techniques to influence the rational design and engineering of templated materials, which will influence a broad number of material directives, advance innovative technologies and discovery, and lead to better integration of imprinted structures in technologies to benefit society. . Create a multi-level, diverse, integrated research team consisting of the PI, 2 under-represented or minority doctoral graduate students, and 3 under-represented or minority undergraduate researchers with a focus on broadening the participation of under-represented groups in research opportunities. . Provide multi-disciplinary, collaborative research training and education for 2 graduate and 3 undergraduate students by integrating polymer science, polymer engineering, materials engineering, molecular recognition, and chemical engineering principles into a research and educational project. . Increase the excitement for undergraduate students toward research activities and careers by integrating state of the art research and discovery in polymer engineering with educational activities. . Broadly disseminate results by publishing results in diverse media (polymer science and engineering journals, biomaterials science journals, drug delivery journals) as well as conference presentations to a diverse number of societies (Materials Research Society, Society for Biomaterials, American Institute for Chemical Engineers). . Incorporate transformative technologies and research from this work in the classroom within the PI's courses. . Increase public understanding and stimulate K-12 interest in science and engineering by incorporating the impact of this work in a "Polymer Show" for Auburn's TIGER Summer Camp. . Provide mentoring, professional development, and career guidance to graduate and undergraduate students, which will help build a well-prepared workforce. . Promoting faculty development through the graduate and undergraduate research mentoring experience.