Electrochemical Detection of Drug Metabolites based on Molecularly Imprinted Polymer Magnetic Nanoparticles

基于分子印迹聚合物磁性纳米粒子的药物代谢物电化学检测

• 批准号: 10372143
• 负责人: Percy Calvo Marzal
• 金额: $ 7.5万
• 依托单位: UNIVERSITY OF CENTRAL FLORIDA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10372143
• 关键词: American; Area; Cessation of life; Chemicals; Clinical; Cocaine; Decentralization; Detection; Discrimination; Drug Adulterations; Drug Controls; Electrochemistry; Electrodes; Emergency Situation; Ensure; Epidemic; Fentanyl; Forensic Sciences; Goals; Heroin; Illicit Drugs; Immunoassay; Ingestion; Laboratories; Location; Magnetic nanoparticles; Magnetism; Mass Fragmentography; Medical; Methacrylates; Methamphetamine; Methods; Modification; Molecular; Morphine; Opioid; Overdose; Parents; Performance; Pharmaceutical Preparations; Polymers; Process; Provider; Reaction Time; Recording of previous events; Regulation; Reproducibility; Research; Research Project Grants; Safety; Sampling; Schedule; Site; Specificity; Structure; Surface; Technology; Testing; United States National Institutes of Health; Urine; Validation; analog; base; cross reactivity; design; detection limit; detection platform; drug misuse; drug testing; economic cost; experience; first responder; imprint; innovation; opioid epidemic; programs; rapid detection; sensor; sensor technology; success; synthetic drug; synthetic opioid; user-friendly

PROJECT SUMMARY Today, 100 Americans die every day from drug overdose impacting the economy at ~100 billion dollars per year. This number of deaths is attributed mainly to the introduction of new synthetic opioids like fentanyl and its analogs. The primary issue with synthetic drugs is that as the parent drug comes under the regulation act, new analogs will appear on the market to circumvent drug controls. In fact, fentanyl suffers a rapid modification and it is hard to keep ahead of or even in front of the drug marker to inform medical providers and first responders of what kind of drugs was ingested. Further, fentanyl and its analogs have been used as a cutting agent to adulterate drugs (e.g. cocaine, heroin, morphine, and methamphetamine) on the street. The goal of this project is to develop, optimize and validate a sensor based on molecularly imprinted polymer (MIP) and magnetic nanoparticles using electrochemical detection for rapid detection of fentanyl analogs’ metabolites. By developing this MIP magnetic sensor, we aim to provide medical providers and first responders with an inexpensive and accurate manner to identify these metabolites on-site. Fentanyl and fentanyl analogs will metabolize to a very few known metabolites with a very similar core structure. Thus, we hypothesize that identification and recognition of this core structure could aid in the detection of existing and new fentanyl analogs. Therefore, the goal of this proposal is to develop a MIP magnetic nanoparticle sensor that recognizes fentanyl analog metabolites with high sensitivity and selectivity. The high sensitivity will be possible by using MIP magnetic nanoparticles allowing pre-concentration of the analyte on the electrode surface. The high selectivity will be possible by using MIP, which will form a unique cavity for the recognition of the core structure of the chosen metabolite. To accomplish this goal, we will i) develop, optimize and characterize a MIP magnetic-sensor for the detection of fentanyl analog’ metabolites; and ii) apply the MIP magnetic-sensor for detection of fentanyl analogs’ metabolites in urine samples and compare with reference methods (GC/MS). The proposed research will produce a new sensor technology for analysis of any fentanyl analogs’ metabolites with the capability to differentiate these powerful drugs from other opioids in urine samples. The resulting sensor is expected to be used in decentralized locations by first responders, clinical laboratories, and medical providers.

项目总结 今天，每天有100名美国人死于药物过量，影响经济，每年约1000亿美元 年。这一死亡人数主要是由于引入了新的合成阿片类药物，如芬太尼和它的 类比。合成药物的主要问题是，随着母药受到管理法的约束，新的 类似药物将出现在市场上，以规避药品管制。事实上，芬太尼经历了快速的修改和 很难保持领先，甚至领先于药物标记物，以告知医疗提供者和急救人员 吃了什么样的毒品。此外，芬太尼及其类似物已被用作切割剂 在街上掺入毒品(如可卡因、海洛因、吗啡和冰毒)。 本项目的目标是开发、优化和验证基于分子印迹聚合物的传感器。 (MIP)和磁性纳米粒子的电化学检测用于快速检测芬太尼类似物 代谢物。通过开发这种MIP磁性传感器，我们的目标是为医疗提供者和急救人员提供 以一种廉价和准确的方式现场鉴定这些代谢物。芬太尼和芬太尼类似物 会代谢成极少数具有非常相似核心结构的已知代谢物。因此，我们假设 对这种核心结构的识别和识别可以帮助检测现有的和新的 芬太尼类似物。因此，这项提议的目标是开发一种MIP磁性纳米颗粒传感器， 识别芬太尼类似代谢物，具有高灵敏度和选择性。高灵敏度将是可能的 通过使用MIP磁性纳米颗粒，可以在电极表面预浓缩分析物。这个 使用MIP将有可能实现高选择性，这将形成用于识别芯子的独特空腔 所选代谢物的结构。 为了实现这一目标，我们将：1)开发、优化和表征一种用于检测的MIP磁传感器 芬太尼类似物的代谢产物；以及ii)应用MIP磁性传感器检测芬太尼类似物。 尿样中的代谢物，并与参考方法(GC/MS)进行比较。 这项拟议的研究将产生一种新的传感器技术，用于分析任何芬太尼类似物。 能够在尿样中将这些强效药物与其他阿片类药物区分开来的代谢物。这个 所产生的传感器预计将在分散的地点由急救人员、临床实验室和 医疗服务提供者。