Electrochemical Detection of Drug Metabolites based on Molecularly Imprinted Polymer Magnetic Nanoparticles

项目摘要 今天，每天有100名美国人死于药物过量，每年影响经济约1000亿美元。 年这一死亡人数主要归因于新合成阿片类药物的引入，如芬太尼及其 类似物合成药物的主要问题是，由于母体药物受监管法案的约束， 类似物将出现在市场上，以规避药物管制。事实上，芬太尼会迅速被改造， 很难保持领先甚至领先于药物标记，以告知医疗提供者和第一反应者 摄入了什么样的毒品此外，芬太尼及其类似物已被用作切割剂， 在街上贩卖毒品（如可卡因、海洛英、吗啡及甲基安非他明）。 本计画的目标是发展、优化及验证一种以分子印迹聚合物为基础的感测器 (MIP)和磁性纳米颗粒用于快速检测芬太尼类似物 代谢物。通过开发这种MIP磁传感器，我们的目标是为医疗提供者和急救人员提供 以廉价和准确的方式现场鉴定这些代谢物。芬太尼和芬太尼类似物 将代谢成极少数具有非常相似核心结构的已知代谢物。因此，我们假设， 这种核心结构的鉴定和识别可以帮助检测现有的和新的 芬太尼类似物。因此，本提案的目标是开发MIP磁性纳米颗粒传感器， 以高灵敏度和选择性识别芬太尼类似物代谢物。高灵敏度将成为可能 通过使用MIP磁性纳米颗粒，允许在电极表面上预浓缩分析物。的 通过使用MIP，高选择性将是可能的，MIP将形成用于识别核的独特空腔 所选代谢物的结构。 为了实现这一目标，我们将i）开发，优化和表征MIP磁传感器用于检测 芬太尼类似物的代谢物;和ii）应用MIP磁传感器检测芬太尼类似物的代谢物。 代谢物的尿液样本，并与参考方法（GC/MS）进行比较。 拟议的研究将产生一种新的传感器技术，用于分析任何芬太尼类似物。 这些代谢物具有将这些强效药物与尿样中的其他阿片类药物区分开的能力。的 由此产生的传感器预计将用于分散的地点，由第一响应者，临床实验室， 医疗供应商。

PROJECT SUMMARY Today, 100 Americans die every day from drug overdose impacting the economy at ~100 billion dollars per year. This number of deaths is attributed mainly to the introduction of new synthetic opioids like fentanyl and its analogs. The primary issue with synthetic drugs is that as the parent drug comes under the regulation act, new analogs will appear on the market to circumvent drug controls. In fact, fentanyl suffers a rapid modification and it is hard to keep ahead of or even in front of the drug marker to inform medical providers and first responders of what kind of drugs was ingested. Further, fentanyl and its analogs have been used as a cutting agent to adulterate drugs (e.g. cocaine, heroin, morphine, and methamphetamine) on the street. The goal of this project is to develop, optimize and validate a sensor based on molecularly imprinted polymer (MIP) and magnetic nanoparticles using electrochemical detection for rapid detection of fentanyl analogs’ metabolites. By developing this MIP magnetic sensor, we aim to provide medical providers and first responders with an inexpensive and accurate manner to identify these metabolites on-site. Fentanyl and fentanyl analogs will metabolize to a very few known metabolites with a very similar core structure. Thus, we hypothesize that identification and recognition of this core structure could aid in the detection of existing and new fentanyl analogs. Therefore, the goal of this proposal is to develop a MIP magnetic nanoparticle sensor that recognizes fentanyl analog metabolites with high sensitivity and selectivity. The high sensitivity will be possible by using MIP magnetic nanoparticles allowing pre-concentration of the analyte on the electrode surface. The high selectivity will be possible by using MIP, which will form a unique cavity for the recognition of the core structure of the chosen metabolite. To accomplish this goal, we will i) develop, optimize and characterize a MIP magnetic-sensor for the detection of fentanyl analog’ metabolites; and ii) apply the MIP magnetic-sensor for detection of fentanyl analogs’ metabolites in urine samples and compare with reference methods (GC/MS). The proposed research will produce a new sensor technology for analysis of any fentanyl analogs’ metabolites with the capability to differentiate these powerful drugs from other opioids in urine samples. The resulting sensor is expected to be used in decentralized locations by first responders, clinical laboratories, and medical providers.