Analysis of novel plastid proteins targeting nucleic acids: Identification of native RNA/DNA ligands and interacting proteins

叶绿体编码一小部分蛋白质，这些蛋白质对光合复合体的生物发生和基因表达机制非常重要。它们的表达需要多种核编码因子。最近，我们发现了两个参与质体基因表达的新蛋白。PTAC12是一种功能未知的蛋白质，但已被发现是转录活性染色体的组成部分，表明其在转录或转录后发挥作用。PPR10是一种五肽重复蛋白，作为RNA结合蛋白参与光合作用基因的RNA稳定和成熟。本项目旨在利用分子和生物化学工具阐明PTAC12的生理功能，在全基因组范围内鉴定其天然核酸配体，并通过氧化还原信号分析其潜在的环境光调节作用。基于蛋白质组学的方法侧重于鉴定PTAC12和PPR10的潜在蛋白质相互作用伙伴。新的蛋白质将在植物中被表征。该项目旨在促进对DNA/RNA代谢过程的理解，并深入了解参与质体基因表达的新蛋白质的作用。

Chloroplasts encode a small set of proteins which are however important for the biogenesis of photosynthetic complexes and the gene expression machinery. Their expression requires a variety of nuclear encoded factors. Recently, we have identified two novel proteins involved in plastid gene expression. PTAC12 is a protein of unknown function but has been discovered as component of the transcriptionally active chromosome suggesting a role in transcription or post-transcription. PPR10, a pentatricopeptide repeat protein, acts as RNA binding protein which is involved in RNA stabilization and maturation of photosynthesis genes. Using molecular and biochemical tools the project aims to elucidate the physiological function of PTAC12, to identify its native nucleic acid ligands on a genome-wide scale and to analyze its potential environmental light regulation via redox signals. A proteomic-based approach focuses on the identification of potential protein interaction partners of both PTAC12 and PPR10. New proteins will then be characterized in planta. The project aims to advance understanding of DNA/RNA metabolic processes and to achieve insights into the role of new proteins involved in plastid gene expression.