Motility Contrast for Endogenous Multi-functional Imaging of Tissue

该提案将开发第一个应用于三维组织的三维运动性测定，其保留了体内组织的相关分子信号，但具有体外工作的优点。为了实现这一目标，他们将使用运动性对比成像（MCI），这是一种新的短相干成像方法，可将亚细胞纳米级运动检测为宏观动态散斑，作为一种新的内源性造影剂。虽然分子水平的功能成像通常需要标签或报告分子，但与运动性相关的功能将提供不需要标签的多探针功能成像方法。这种内源性多功能成像是本提案的长期目标。拟议的工作是在组织为基础的药物筛选的背景下，在体外进行，未来可能在体内扩展。该提案强调直接影响细胞运动的细胞骨架和抗有丝分裂药物。

0756005NolteThis proposal will develop the first three-dimensional motility assays applied to three dimensional tissues that retain the relevant molecular signaling of in vivo tissue but with the advantages of working in vitro. To accomplish this objective they will use motility contrast imaging (MCI), which is a new short-coherence imaging approach that detects sub-cellular nanoscale motion as macroscopic dynamic speckle that acts as a novel endogenous contrast agent. While functional imaging at the molecular level usually requires tags or reporter molecules, function associated with motility would provide a multi-probe functional imaging approach that requires no tags. This endogenous multi-functional imaging is the long-range goal of this proposal. The proposed work is in the context of tissuebased drug screening that is performed in vitro with possible future extensions in vivo. The proposal emphasizes cytoskeletal and anti-mitotic drugs that directly influence motility in the cell.