Ultra-Efficient Plasmonic Nanoparticle Markers for Second Harmonic Imaging Microscopy

CBET-0756693，RobinsonCBET-07566693，RobinsonCBET-075666693，RobinsonCBET-075666666，RobinsonCBET-0756666，RobinsonCBET-0为了实现这一目标，研究人员建立了两个目标;第一个目标是使用光刻和化学合成技术制造和表征几种不同类型的SHIM增强纳米颗粒，以获得最大的灵活性。第二个是在生物环境中实际演示SHIM成像和热疗。为了实现这一目标，研究人员将使用双功能交联剂对颗粒施加保护涂层，使其具有生物稳定性和无毒性，并且易于与抗体或配体（如叶酸）缀合，以促进颗粒与靶细胞和组织的选择性结合。研究人员将证明纳米颗粒的生物相容性以及它们通过热疗进行靶向治疗的潜力。

CBET-0756693, RobinsonThe intent of this proposal is to develop and characterize a new class of nanoparticles designed to operate as ultra-bright markers for second harmonic imaging microscopy (SHIM) and potentially for other nonlinear microscopy techniques. In order to accomplish this goal the investigators have established two objectives; the first objective is to fabricate and characterize several different types of SHIM enhanced nanoparticles using both lithographic and chemical synthesis techniques to give maximum flexibility. The second is to actually demonstrate SHIM imaging and hyperthermia in a biological setting. In order to accomplish this aim, the investigators will use bifunctional crosslinkers to apply a protective coating to the particles, making them biologically stable and non-toxic, and also easy to conjugate with an antibody or ligand (such as folic acid) to promote selective binding of the particles to target cells and tissues. The investigators will demonstrate the biocompatibility of the nanoparticles as well as their potential to perform targeted therapy through hyperthermia.