Collaborative Research: Biomagnetic Glasses: Preparation, Characterization and Biosensor Applications

电极导线ID：DMR/BMAT（7623）0804464 主要研究者：Stanciu、Lia ORG：Purdue非电极导线ID：DMR/BMAT（7623）0804506 非主要研究者：Andreescu，Emanuela ORG：Clarkson标题：合作研究：生物磁性眼镜：制备、表征和生物传感器应用智力优势：本提案的目标是创建一种通用的方法来制造具有磁性能力的生物功能材料，即所谓的“生物磁性玻璃”。“这些将用于设计高度稳定的、磁性可分离的酶基系统。该项目的重点是创建包含酶，磁性纳米颗粒和二氧化硅玻璃的混合复合材料，以及开发用于制备这些材料的通用策略。 三种酶已被选为模型的例子：辣根过氧化物酶（HRP），乙酰胆碱酯酶（AChE），葡萄糖-6-磷酸脱氢酶（G6 PDH）。 作为所提出的应用的一个例子，基于乙酰胆碱酯酶的生物磁性玻璃将适合于用作开发用于检测食品、农业领域和其他安全情况中的农药的廉价且自主操作的生物分析装置中的感测组件。 有人提出，传感器材料的磁性将允许它们相对于使用永磁体的潜在分析物被定位。 本项目的具体目标是：（1）合成和表征具有可控微结构和性能的二氧化硅-氧化铁磁性纳米粒子。（Purdue）;（2）包封模型酶（HRP，AChE，G6 PDH）在二氧化硅磁性纳米复合材料中的生物催化活性和稳定性（Purdue）;（3）使用丝网印刷电极开发生物磁性玻璃在生物传感器中的应用（克拉克森）;（4）在应用各种浓度的目标分析物时建立生物传感器的校准图（例如过氧化氢、杀虫剂、葡萄糖-6-磷酸）（克拉克森）。更广泛的应用：在生物技术工艺流、环境和安全领域中的化学物质分析中，仍然非常需要在宽动态范围内具有灵敏度和选择性的基于生物的传感器技术。 该提案特别解决了基于酶的生物传感器的使用寿命问题。 该项目为研究生和本科生的多学科培训提供了一个很好的平台。 两个PI都鼓励本科生参与;支持本科生的资金包括在克拉克森预算中。 普渡大学的PI计划将生物传感器的材料纳入她在两所学校教授的课程中，她是其中的一员。 这两个PI都将与当地公立学校进行外联。 在克拉克森，这些关系已经建立并正在进行中，而普渡大学的PI正在与学校系统建立必要的关系。 她还积极参与普渡大学的女性工程项目，并成功地招募了女学生加入她的研究小组。 这两个PI都表现出对扩大代表性不足的少数民族学生参与的敏感性。 PI与各自校园内正在进行的少数族裔招聘计划有关。

Lead ID: DMR/BMAT(7623) 0804464 Lead PI: Stanciu, Lia ORG: PurdueNon-Lead ID: DMR/BMAT(7623) 0804506 Non-Lead PI: Andreescu, Emanuela ORG: ClarksonTitle: COLLABORATIVE RESEARCH: Biomagnetic Glasses: Preparation, Characterization, and Biosensor ApplicationsINTELLECTUAL MERIT: The goal of this proposal is to create a generic approach for the fabrication of biofunctional materials with magnetic capabilities, so called "biomagnetic glasses." These will be used in the design of highly stable, magnetically separable enzyme-based systems. The project focuses on creating hybrid composite materials containing enzymes, magnetic nanoparticles, and silica glasses and on the development of generic strategies that will be used to prepare these materials. Three enzymes have been selected as model examples: horseradish peroxidase (HRP), acetylcholinesterase (AChE), and glucose-6-phosphate dehydrogenase (G6PDH). As an example of proposed applications, the AChE based biomagnetic glasses will be suitable for use as the sensing component in the development of a cheap and autonomously operating bioanalytical device for detecting pesticides in food products, agricultural fields and other security situations. It is proposed that the magnetic properties of the sensor materials will permit them to be localized with respect to the potential analytes using permanent magnets. The specific objectives of the project are: (1) synthesize and characterize silica-iron oxide magnetic nanoparticles with controlled microstructure and properties. (Purdue); (2) encapsulate model enzymes (HRP, AChE, G6PDH) within the silica magnetic nanocomposites and monitor the biocatalytic activity and stability (Purdue); (3) use screen printed electrodes to exploit the biomagnetic glasses in biosensors (Clarkson); (4) build calibration plots of the biosensors upon application of various concentrations of target analytes (e.g. hydrogen peroxide, pesticides, glucose-6-phosphate) (Clarkson).BROADER IMPACTS: Bio-based sensor technology that is sensitive and selective across a broad dynamic range remains in great demand for analysis of chemical species in biotech process streams, in the environment, and in the security area. This proposal addresses, in particular, the issue of useful lifetime of enzyme-based biosensors. The project provides an excellent platform for multidisciplinary training of graduate and undergraduate students. Both PIs encourage undergraduate participation; funding for support of undergraduates is included in the Clarkson budget. The Purdue-based PI plans to incorporate materials on biosensors in courses that she teaches in the two Schools of which she is a member. Both PIs will engage in outreach to local public schools. At Clarkson these relationships are established and on-going, while the PI at Purdue is developing the necessary relationships with the school system. She is also active in the Purdue Women in Engineering program and has been successful in recruiting female students into her research group. Both PIs demonstrate a sensitivity toward broadening participation among underrepresented minority students. The PIs are connected with ongoing minority recruitment programs on their respective campuses.