Collaborative Research: CPSF30 at the Convergence of RNA Processing, Cellular Signaling, and Development in Plants

亚瑟·G·亨特和Q.Quinn Li IOS-0817818合作建议：CPSF30在RNA处理、细胞信号传递和植物发育的融合基因表达在许多水平上受到控制，包括RNA的处理。关于环境输入(刺激)导致RNA加工变化的机制，仍有许多问题。本项目的研究涉及一种可能的机制，涉及一种RNA加工因子(CPSF30)在植物基因表达调控中的作用。这项工作的灵感来自先前的观察，即不产生这种蛋白质的突变植物具有一系列有趣的表型，而且这种蛋白质本身受到细胞感觉系统的一个中心组成部分的调节。这个项目将结合遗传学、生化、分子和系统的方法来理解细胞信号和CPSF30功能之间的相互作用。该项目的研究部分的成果将是更好地了解刺激可以导致植物细胞中RNA处理的变化的方式。这反过来将有助于更好地了解植物对环境线索和致病有机体的反应方式。这个项目的影响将远远超出对植物基因表达的理解。预期的结果将有助于开发新的遗传和生物技术战略，以改善作物在不利条件下的表现。该项目将涉及博士后科学家、研究生和本科生。这个项目具有明确的跨学科性质，因为它涉及分子/生化、全植物和系统生物学方法。因此，这些研究将有助于培训人员为在日益跨学科的科学领域(植物生物学)做出贡献做好准备。参与者(包括私人投资者)将参与社区计划，以促进公众对科学的兴趣和欣赏。

Arthur G. Hunt and Q. Quinn Li IOS-0817818Collaborative Proposal: CPSF30 at the convergence of RNA processing, cellular signaling, and development in plantsGene expression is controlled at many levels, including the processing of RNAs. There remain many questions as to the mechanisms by which environmental inputs (stimuli) lead to changes in RNA processing. The research to be pursued in this project deals with one possible mechanism, involving the role of one RNA processing factor (CPSF30) in regulated gene expression in plants. This work is inspired by prior observations that mutant plants that do not make the protein have an interesting range of phenotypes, and that the protein itself is regulated by a central component of cellular sensory systems. This project will combine genetic, biochemical, molecular, and systems approaches to understand the interplay between cellular signaling and the function of CPSF30. The outcome of the research component of the project will be a better understanding of the ways in which stimuli can cause changes in the processing of RNAs in the plant cell. This will in turn contribute to a better understanding of the ways by which plants respond to environmental cues and disease-causing organisms. This project will have an impact that extends significantly beyond an understanding of gene expression in plants. The expected results will be of help in developing new genetic and biotechnological strategies for improving the performance of crop plants in adverse conditions. This project will involve postdoctoral scientists, graduate students, and undergraduate students. There is a decided cross-disciplinary nature to this project, in that it involves molecular/biochemical, whole-plant, and systems biology approaches. As such, these studies will help to prepare trainees to make contributions in a scientific field (plant biology) that is becoming more cross-disciplinary. Participants (including PIs) will participate in community programs so as to promote interest in and appreciation of science in the general public.