MRI: Acquisition of a Laser Scanning Confocal Microscope to Advance Research and Research Training Opportunities at San Francisco State University

一笔赠款已授予旧金山弗朗西斯科州立大学（SFSU）的监督下，戴安娜朱博士购买激光扫描共聚焦显微镜的细胞和分子成像中心（CMIC）。该仪器包括高效和灵敏的光谱检测器，可以分析荧光标记样品的高分辨率，三维重建。这将推动研究，揭示胚胎发育机制，使用非洲爪蟾和鸡，细胞内信号在植物中，和癌症治疗化合物的设计。多个激光器和对激发和发射波长的精确控制将使同时使用广泛的荧光团成为可能，这些荧光团是了解蠕虫中染色质功能、蚂蚁和螃蟹中基因表达以及哺乳动物中染色体定位所需的。现场访问将允许实时成像捕捉C中的快速染色体运动。elegans和鸡肌肉发育中的瞬时细胞信号。该仪器的灵敏度，分辨率和速度将允许SFSU研究人员采用先进的成像技术，以解决在发育生物学，化学和数学各自领域的前沿新的研究问题。SFSU教师的研究行为将与使用该系统的密切指导培训相结合，以促进SFSU学生的职业发展，其中35%是代表性不足的少数民族和58%是女性。这种多样性反映在本提案中包括的11名调查员中，其中包括5名妇女、3名拉丁美洲人和1名美洲土著人。在荧光和共聚焦显微镜的个人研究项目和课程将准备在博士机构和生物技术公司的成功学生。负担得起的访问这个系统也将加强在邻近机构的研究人员和SFSU的不同教师和学生之间的合作。

A grant has been awarded to San Francisco State University (SFSU) under the supervision of Dr. Diana Chu to purchase a Laser Scanning Confocal Microscope for the Cell and Molecular Imaging Center (CMIC). The instrument includes efficient and sensitive spectral detectors to allow analysis of high-resolution, 3-dimensional reconstructions of fluorescently-labeled samples. This will advance studies that uncover mechanisms of embryonic development using Xenopus and chick, intracellular signaling in plants, and the design of cancer therapy compounds. Multiple lasers and precise control over excitation and emission wavelengths will enable the simultaneous use of a broad range of fluorophores that are required to understand chromatin function in worms, gene expression in ants and crabs, and chromosome positioning in mammals. On-site access will allow live imaging to capture rapid chromosome movements in C. elegans and transient cell signals in chick muscle development. The sensitivity, resolution, and speed of this instrument will permit SFSU researchers to employ advanced imaging techniques to address new research questions at the forefront of their respective fields in developmental biology, chemistry and mathematics. The research SFSU scholar-teachers conduct will be integrated with closely mentored training using this system to enhance the career development of SFSU students, 35% of whom are under-represented minorities and 58% women. This diversity is reflected in the 11 investigators included in this proposal, which includes 5 women, 3 Latinos, and 1 Native American. Individual research projects and courses in fluorescence and confocal microscopy will prepare students for success at PhD institutions and biotechnology companies. Affordable access to this system will also enhance collaborations between researchers at neighboring institutions and the diverse faculty and students at SFSU.