Opto-Fluidic Hybrid System for Miniaturized Flow Cytometry

本研究的目的是开发一种光-流混合小型化流式细胞仪。 该方法是实现集成的、完全功能化的片上系统，其能够（1）细胞的三维（3D）聚焦，（2）在X-Y和X-Z平面中对光的灵活操纵，以及（3）同时收集所有三种类型的光信号（前向散射、侧向散射和荧光）。该研究将解决现有小型化流式细胞仪芯片中的两个主要问题：缺乏垂直（Z）方向的流动聚焦和缺乏光的片上聚焦。 它的功能和简单的制造和组装将使所提出的系统的基础生物医学研究和临床应用的吸引力。 更广泛的影响除了技术上的贡献外，该项目还将通过实施与拟议研究紧密结合的、面向各级群体（普通公众、本科生和研究生）的积极教育和推广计划，对社会产生影响。 代表性不足的学生将广泛参与拟议的研究，并通过宾夕法尼亚州立大学WISER和MURE计划招募。 在该项目过程中取得的成果和发展的专门知识将纳入PI的教学活动。为了获得尽可能广泛的影响，PI将与宾夕法尼亚州立大学MRSEC合作，开发一套MEMS/NEMS演示，并将其提供给公众。

ObjectiveThe objective of this research is to develop an opto-fluidic hybrid miniaturized flow cytometry. The approach is to implement an integrated, fully functionalized on-chip system, which is capable of (1) three-dimensional (3D) focusing of cells, (2) flexible manipulation of light in both X-Y and X-Z planes, and (3) simultaneous collection of all three types of light signals (forward scattering, side scattering, and fluorescence). Intellectual MeritThe proposed research will address two major problems in the existing miniaturized flow cytometry chips: lack of flow focusing in the vertical (Z) direction and lack of on-chip focusing of light. Its functionality and simplicity of fabrication and assembly will make the proposed system attractive for both fundamental biomedical research and clinical applications. Moreover, it will shed light on our understanding in the light and fluid interactions at microscale, providing momentum to the emerging field of optofluidics.Broader ImpactIn addition to its technical contributions, the project will impact society by implementing vigorous education and outreach programs that are closely integrated with the proposed research and designed for groups at all levels (general public, undergraduates, and graduate students). Underrepresented students will participate extensively in the proposed research and be recruited through the Penn State WISER and MURE programs. Results and expertise developed during the course of this project will be incorporated into the PI's teaching activities. To obtain the broadest possible impact, the PI will partner with the Penn State MRSEC to develop a suite of demonstrations in MEMS/NEMS and deliver them to the general public.