Engineering an efficient biocatalyst for chiral compound production

这项计划将开发新型高效的辅因子再生细菌菌株，用于手性化合物的全细胞生物转化，手性化合物通常用作合成重要药物的中间体。该项目将研究利用基本细胞代谢途径的修饰来再生辅因子的想法，从而将工程生物过程与整体细胞生理学相结合。布罗德影响有效再生辅因子的能力将对许多过程产生影响。此外，本工作中开发的框架可应用于生产许多需要辅因子NADPH进行生物合成的生化产品的其他系统。这些项目将为研究生和本科生提供培训机会。参与这类研究将为研究生提供生化和代谢工程、生物系统工程、生物化学和分子生物学方面的跨学科培训。

CBET-0828516Ka-Yiu San, William Marsh Rice UniversityIntellectual merit This project will lead to the development of novel and efficient cofactor regeneration bacterial strains for whole cell biotransformation of chiral compounds, which are often used as intermediates in the synthesis of important pharmaceutical compounds. The project will investigate the idea of using modification of essential cell metabolic pathways for regeneration of the cofactor and thus the integration of the engineered bioprocess with overall cell physiology.Broader impacts The ability to regenerate cofactor efficiently will have impacts on a number of processes. In addition, the framework developed in this work could be applied to other systems for the production of many biochemical products that require the cofactor NADPH for their biosynthesis. The projects will provide training opportunities for graduate and undergraduate students. Participation in this type of research will provide graduate students with interdisciplinary training in biochemical and metabolic engineering, biosystems engineering, biochemistry and molecular biology.