Rapid, Sensitive and Sequential Detection of E. coli and Total Coliforms Using Engineered Conducting Membranes for Water Quality Control

本文的主要目的是开发一种快速、易用、灵敏的总大肠菌群和大肠杆菌检测系统。该方案的思想优点包括：首先，两种工程导电膜（聚吡咯和单壁碳纳米管）将过滤水样，从而作为细菌浓缩器，实现了细菌在聚吡咯和SWNT上的快速浓缩和固定化；其次，导电聚吡咯和SWNT膜也可以作为安培检测的工作电极，由于其超电子导电性、抗污染性能和强电催化能力，提高了灵敏度；第三，分别为<s:1> - d -半乳糖苷酶（总大肠菌群）和<e:1> - d -葡萄糖醛酸酶（大肠杆菌）选择/鉴定了两种“电致”底物，酶解反应的水解产物具有可区分的氧化电位，从而使大肠杆菌和总大肠菌群的顺序检测成为可能。这些特点将使该检测系统成为饮用水水质控制的理想分析工具。该计划为本科生和研究生提供了一个真正的多学科培训基地。预期将与现有的资助项目产生协同效应。这项工作将促进水质控制生物传感器的发展，如果成功，可能对总大肠菌群和粪便指标的检测能力产生巨大影响。研究活动的多学科性质使其适合在非技术期刊上传播，并作为外联活动的主题。该提案包括全年的本科生研究项目，并将成为康涅狄格大学“前沿杰出讲师”计划和康涅狄格大学？“达芬奇计划”（http://www.engr.uconn.edu/davinci/）旨在加强K-12教育。

CBET- 0828627Lei, YuUniversity of ConnecticutRapid, Sensitive and Sequential Detection of E. coli and Total Coliforms Using Engineered Conducting Membranes for Water Quality ControlThe principal objective of this proposal is to develop a fast, easy-to-use, and sensitive detection system for total coliforms and E. coli. The intellectual merits of this proposal include: First, two engineered conducting membranes (polypyrrole and single-walled carbon nanotubes) will filter water sample and, thus, serve as bacteria concentrator, achieving the quick concentration and immobilization of bacteria on polypyrrole and SWNT in a single step; Second, the conducting polypyrrole and SWNT membranes also serve as working electrodes in amperometric detection and enhances the sensitivity due to their super-electron conductivity, anti-fouling property, and strong electrocatalytic capability; Third, two "electrogenic" substrates are specifically selected/identified for â-D-galactosidase (total coliforms) and â-D-glucuronidase (E. coli) respectively, and the hydrolyzed products from enzymatical reactions have distinguishable oxidation potentials, allowing the sequential detection of E. coli and total coliforms possible. All these features will make the proposed detection system an ideal analytical tool for drinking water quality control. The proposed program offers a truly multidisciplinary training ground for undergraduates and graduate students. Synergistic effects with existing grants to the PI are expected. The work will advance the development of biosensor for water quality control in general and, if successful, might have an enormous impact on the ability to detect total coliforms and fecal indicator. The multidisciplinary nature of the research activity lends itself for dissemination in non-technical journals and as a subject of outreach activities. The proposal includes year-round undergraduate research projects and will be part of UConn "Frontiers Distinguished Lectureship" program and UConn?s School of Engineering Da Vinci project (http://www.engr.uconn.edu/davinci/) to enhance K-12 education.