MRI: Acquisition of a multi-photon imaging and electrophysiology rig

该奖项是根据2009年《美国复苏和再投资法案》(公法111-5)提供资金的。显微镜和高分辨率荧光成像的结合使用是探索物理和生物世界的基本工具。来自NSF的这笔重大研究仪器拨款为达特茅斯社区提供了一种最先进的多光子显微镜设施，将推动三大科学研究路线。第一个研究领域是探索哺乳动物中枢神经系统的基本特性，包括神经发育和神经信号转导，在体外和体内。第二条线的研究正在研究细胞生物学的基本方面，包括细胞信号和细胞内蛋白质的运输。第三条研究途径将探索多光子成像在生物和非生物应用中的新用途。所有这些研究领域都得益于多光子成像固有的量子效率的提高和光毒性和光漂白的减少。美国国家科学基金会提供的多光子成像设备极大地增强和扩展了达特茅斯多个实验室的研究能力，并正在促进不同用户群体之间的新的合作研究项目。新的发现将通过期刊出版物、会议报告和当地媒体传播。除了促进研究外，多光子成像设施还促进了对下一代科学研究人员的教育和培训。该机构的200多个实验室提供了先进光学技术培训，其中包括250多名研究生。这一新仪器的使用增加了达特茅斯大学研究生和博士后研究员的研究机会和经验。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). The combined use of microscopy and high-resolution fluorescence imaging is a fundamental tool for exploration of the physical and biological world. This Major Research Instrumentation grant from the NSF provides the Dartmouth community with a state-of-the-art multi-photon microscopy facility that will drive three broad lines of scientific inquiry. The first research area is exploring fundamental properties of the mammalian central nervous system, including neural development and neuronal signaling, in vitro and in vivo. A second line of research is investigating fundamental aspects of cellular biology, including cellular signaling and intracellular trafficking of proteins. A third avenue of research will explore novel uses for multi-photon imaging for biological and non-biological applications. All of these research areas benefit from the increased quantum efficiency and reduced phototoxicity and photo-bleaching that are inherent in multi-photon imaging. The NSF-provided multi-photon imaging facility greatly enhances and expands the research capabilities of a broad range of laboratories at Dartmouth, and is fostering new collaborative research projects among a diverse group of users. New findings will be disseminated through journal publications, conference presentations, and local media. In addition to facilitating research, the multi-photon imaging facility is advancing the education and training of the next generation of scientific investigators. Training in advanced optical techniques is made available to more than 200 laboratories across the institution, including more than 250 graduate students. Access to this new instrumentation increases the research opportunities and experiences available to Dartmouth graduate students and postdoctoral fellows.