Analysis of Radial Patterning in Arabidopsis

PI：Frans E.税收提案编号：IOS-0922678标题：拟南芥中的径向图案化分析该奖项由2009年美国复苏和再投资法案(公法111-5)资助。径向图案化是所有植物发展中的一个基本过程。根、茎和花呈现放射状的组织模式，这种模式是在早期胚胎中形成的。这个项目的目标是了解植物组织是如何组织成同心的细胞层的。由于细胞层的产生预计需要细胞之间的沟通，因此当细胞层首次建立时，受体中的突变将被预测为影响胚胎中的放射状模式。在模式植物拟南芥中，发现两个受体的双突变可以将胚胎中的外部细胞类型转变为内部细胞类型。在这个项目中，将检验胚胎外层作为组织者并调节内层身份的假设。利用遗传和生化方法，这些受体和作用于这些受体的蛋白质的候选信号将被表征。这个项目将提供一个框架，用于理解植物中负责径向花纹形成的信号机制，目前还没有很好的理解。本项目的研究结果可用于调控作物的茎大小、根生长和雄性不育等重要性状。该项目将有助于对研究生、本科生、高中生和教师进行植物发育、遗传学和基因组学方面的培训。第一个暑假将招聘一名教师来开发植物遗传学或基因组学单元，并在学年期间实施该单元，并在第二个暑假完善该单元。

PI: Frans E. Tax Proposal number: IOS-0922678Title: Analysis of Radial Patterning in ArabidopsisThis award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)Radial patterning is a fundamental process in the development of all plants. Roots, stems, and flowers exhibit a radial pattern of organization, and this pattern is formed in the early embryo. The goal of this project is to understand how plant tissues become organized as concentric layers of cells. Since the generation of layers of cells is expected to require communication between cells, mutants in receptors would be predicted to affect radial pattern in embryos, when layers of cells are first established. Double mutants for two receptors in the model plant Arabidopsis were found to change outer cell types to inner cell types in the embryo. In this project, the hypothesis that the outer layer of the embryo acts as an organizer and regulates the identity of inner layers will be tested. Using genetic and biochemical approaches, candidate signals for these receptors and proteins that these receptors act on will be characterized. This project will provide a framework for understanding the signaling mechanisms responsible for radial patterning in plants, which is currently not well understood. The results from this project can be applied to regulating important traits such as stem size, root growth and male sterility in crop plants. This project will contribute to the training of graduate students, undergraduates and high school students and teachers in plant development, genetics and genomics. A teacher will be recruited the first summer to develop a plant genetics or genomics-based unit, will implement the unit during the school year, and refine it during a second summer.