Arabidopsis 2010: Analysis of Four Families of Receptor Protein Kinases

拟南芥 2010：四个受体蛋白激酶家族的分析

• 批准号: 0418946
• 负责人: Frans Tax
• 金额: --
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0418946&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; Analysis; Four; Families

The receptor-like protein kinases (RLKs) are the largest class of Arabidopsis protein kinases. This class, which based on phylogenetic analysis contains both transmembrane and cytoplasmic protein kinases, consists of at least 610 members and thus represents about 2.5% of the protein encoding genes in Arabidopsis . 417 of the RLK genes contain a putative ligand binding domain, a single transmembrane domain and a predicted cytoplasmic protein kinase domain, and thus are predicted to function as receptors. These 417 genes can be classified into 21 different types based on the sequences found in their extracellular domains. However, there is currently functional information on fewer than a dozen of the transmembrane RLKs. This research will focus on 61 of the 216 RLK genes that contain extracellular leucine-rich repeats (LRRs). Six well characterized RLK mutants in Arabidopsis thaliana :erecta, clavata1, brassinosteroid insensitive1, excess microsporocytes1, vascular highway1, and haesa belong to the LRR VII, X, XI and XII families. Based on what is known about the functions of these six well characterized LRR RLKs it is reasonable to hypothesize that many of the uncharacterized LRR VII, X, XI and XIII genes are likely to play interesting and important roles in plant growth and development. Two emerging themes are: that receptor kinases are part of a cellular network of regulatory proteins that includes physical interactions with other RLKs, and that some receptor kinases are involved in similar or overlapping processes. Using existing tools and technologies, the biological functions of each gene will be determined by isolation and characterization of both loss-of-function and gain-of-function mutations and examination of expression of the LRR VII, X, XI and XIII genes. The regulatory networks that mediate signaling by these protein kinases will also be defined.Network of genes: The figure on the URL :http://www.mcb.arizona.edu/tax/login.cfm, shows a phylogenetic tree of the LRR RLKs in this project and a short description of the project. This will be linked to Frans Tax 's website. There will be links to this from TAIR, PlantsP and the websites from the other participants in the project.Definition of determining the function: The function of these RLKs will be determined through the analysis of the phenotypes of single and multiple mutants and by analysis of the networks these RLKs participate in through results from the yeast two-hybrid system. Additional information to test for phenotypes will come from analysis of the expression patterns and from the analysis by others on RLK activity under specific environmental conditions.What,how,when and where results will be shared: When single or multiple insertion or TILLING mutants are verified, they will be added to the project website and seeds sent to the stock center. The results of phenotypic screens using Gantlet protocols will be displayed on the website,as well photographs of the promoter:GUS expression patterns. These results will also be linked to PlantsP. All seed, strains and molecular reagents generated by this project will be deposited in the ABRC with a full description. Results from these experiments will also be presented at meetings and submitted for publication in a timely manner.This 2010 project targets a unique set of plant genes of unknown function that are expected to have fundamental roles in the control of plant growth and development. Although a great deal is known about signaling networks in other organisms with completed genomes, relatively little is known about the mechanisms by which plant cells perceive and transduce developmental and environmental signals. The availability of several recently developed tools and technologies provides the means to rapidly advance the understanding of the biological functions of the LRR RLKs .Broader ImpactsThe broader impacts of the project include the partnership with high school teachers to adapt the research materials for high school students participating in the Partnership for Research and Education in Plants (PREP), as well as the development of training workshops for teachers and scientists to prepare them to participate in PREP. This project also provides specific mentoring and training for undergraduate students that are members of underrepresented minority groups.

受体样蛋白激酶(RLKs)是拟南芥中最大的一类蛋白激酶。根据系统发育分析，这一类包含跨膜和细胞质蛋白激酶，至少由610个成员组成，约占拟南芥蛋白质编码基因的2.5%。其中417个RLK基因含有一个可能的配体结合区、一个跨膜区和一个预测的胞浆蛋白激酶结构域，因此被预测作为受体发挥作用。根据在胞外区发现的序列，这417个基因可以分为21种不同的类型。然而，目前关于跨膜RLK的功能信息不到12个。这项研究将集中在216个RLK基因中的61个，这些基因含有胞外富含亮氨酸的重复序列(LRR)。拟南芥中的6个RLK突变体：erecta、clavata1、brassinosterid insensitive1、过剩的小孢子球1、血管通道1和HAESA属于LRR VII、X、XI和XII家族。根据已知的这6个LRR RLK基因的功能，可以合理地推测，许多未被特征化的LRR VII、X、XI和XIII基因可能在植物的生长发育中发挥重要的作用。两个新出现的主题是：受体激酶是调控蛋白细胞网络的一部分，包括与其他RLK的物理相互作用，以及一些受体激酶参与相似或重叠的过程。利用现有的工具和技术，每个基因的生物学功能将通过分离和鉴定功能丧失和功能获得突变以及检查LRR VII、X、XI和XIII基因的表达来确定。基因网络：网址：http://www.mcb.arizona.edu/tax/login.cfm，上的图显示了这个项目中LRR RLK的系统发生树和项目的简短描述。这将链接到法国税务公司的S网站。将有来自tair，PlantsP和该项目其他参与者的网站的链接。确定功能的定义：这些RLK的功能将通过分析单个和多个突变体的表型，以及通过分析这些RLK参与的网络，通过酵母双杂交系统的结果来确定。要测试表型的其他信息将来自对表达模式的分析，以及其他人对特定环境条件下RLK活性的分析。结果将分享什么，如何，何时何地：当单个或多个插入或分蘖突变体得到验证时，它们将被添加到项目网站上，并将种子发送到库存中心。使用Gantlet方案的表型筛选结果将在网站上展示，以及启动子的照片：GUS表达模式。这些结果也将与PlantsP联系起来。所有由该项目产生的种子、菌株和分子试剂都将与完整的描述一起存放在ABRC中。这些实验的结果也将在会议上公布，并及时提交发表。这项2010年的项目目标是一组独特的未知功能的植物基因，预计这些基因将在植物生长和发育控制中发挥基础作用。尽管人们对具有完整基因组的其他生物体中的信号网络了解很多，但对植物细胞感知和传递发育和环境信号的机制知之甚少。最近开发的几种工具和技术的提供提供了迅速促进对LRR RLK生物学功能的理解的手段。广泛影响该项目的更广泛的影响包括与高中教师合作，为参加植物研究和教育伙伴关系(PREP)的高中生改编研究材料，以及为教师和科学家开发培训讲习班，为他们参与准备工作做准备。该项目还为属于代表人数不足的少数群体的本科生提供具体的指导和培训。