MRI: Acquisition of LTQ-Orbitrap High Resolution Mass Spectrometer

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。奖项摘要唐纳德·丹佛斯植物科学中心获得了一笔赠款，用于蛋白质组学和代谢组学研究的高分辨率质谱仪。Danforth中心的蛋白质组学质谱设施（PMSF）将容纳NSF支持的Thermo LTQ Orbitrap MS，Eksigent 2D-nanoLC和Advion Triversa纳米喷雾机器人的收购，以支持Danforth中心，我们的合作机构和合作者的科学家的研究。 随着当前生物学研究的深度和广度不断扩展，检测、识别和表征生物分子的能力仍然是阐明复杂细胞过程的重要组成部分。 高分辨率MS能力对于小分子（代谢物）和大分子（肽/蛋白质）的全面表征至关重要。 本提案支持的项目实例包括：分析蛋白质的脂质翻译后修饰的机制和功能，分析第三代生物燃料系统开发中的代谢通量，分析将在动物和植物王国中研究的各种异喹啉生物碱的形成，以及蛋白质组改变的直接和间接后果，以及转基因罂粟的生物碱代谢组的测定。该项目将最先进的研究和教育完美地结合起来。 它支持本科生和研究生，博士后助理和高级人员在不断增长的质谱分析领域的培训。 这种仪器将促进跨学科研究，从而增加植物蛋白质组学和代谢组学领域的知识。 高分辨率质谱仪所提供的能力将通过在PMSF的安置以及通过中心和参与大学的外联方案而被大量受众获得和利用。 根据丹福思中心的使命，通过利用这种设备获得的知识将提高植物的营养含量，以改善人类健康，增加农业产量，以获得对商品作物的竞争优势，并利用农业研究为农民创造新的和增加的价值，并支持研究，以创造新的生物燃料和生物可再生产品，有助于扩大“不断增长的绿色”倡议，区域和国家影响。 项目成果，包括关于工具的信息和使用工具的项目产生的出版物，将在PMSF网站上提供：http://www.danforthcenter.org/pmsf/。 此外，由这笔资金产生的同行评审出版物将在相应的NSF奖项网站上发布。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Award AbstractA grant has been awarded to the Donald Danforth Plant Science Center to acquire high-resolution mass spectrometry for research in proteomics and metabolomics. The Proteomics & Mass Spectrometry Facility (PMSF) at the Danforth Center will house the NSF-supported acquisition of a Thermo LTQ Orbitrap MS, an Eksigent 2D-nanoLC, and an Advion Triversa nanospray robot to support research of scientists at the Danforth Center, our partner institutions, and collaborators. As the depth and breadth of current biological research continues to expand, the ability to detect, identify, and characterize biomolecules remains an essential component in the elucidation of complex cellular processes. High-resolution MS capabilities are essential for full characterization of both small (metabolite) and large (peptide/protein) molecules. Examples of projects supported though this proposal include: analysis of the mechanism and function of lipid post-translational modification of proteins, the analysis of metabolic flux in the development of third generation biofuel systems, the analysis of the formation of diverse isoquinoline alkaloids that will be studied in both the animal and plant kingdoms, and the direct and collateral consequences of proteome alterations, and the determination of the alkaloid metabolome of transgenic opium poppies.This project seamlessly integrates state-of-the-art research and education. It supports the training of undergraduate and graduate students, postdoctoral associates, and senior personnel in the ever-growing analytical field of mass spectrometry. This instrumentation will promote interdisciplinary research leading to increased knowledge in the areas of plant proteomics and metabolomics. The capabilities afforded by a high resolution mass spectrometer will be accessible to and utilized by a large audience through placement in PMSF and through outreach programs at the Center and participating universities. Knowledge gained through utilization of this equipment will, per the Danforth Center mission, enhance the nutritional content of plants to improve human health, increase agricultural production to gain a competitive edge over commodity crops and harness agriculture research to create new and increased value for farmers, and support research to create new biofuels and biorenewable products that contribute to an expanding "growing green" initiative that will have local, regional and national impacts. Project outcomes, including information regarding the instrumentation and publications resulting from projects using the instrumentation, will be available on the PMSF website: http://www.danforthcenter.org/pmsf/. Additionally, peer-reviewed publications resulting from this funding will be posted on the corresponding NSF award website.