Collaborative: RoL: Molecular patterns for redox sensing and signaling in organismic defense

协作：RoL：有机防御中氧化还原传感和信号传导的分子模式

• 批准号: 2108366
• 负责人: Leslie Hicks
• 金额: $ 19.03万
• 依托单位: University of North Carolina at Chapel Hill
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2108366&HistoricalAwards=false
• 关键词: Collaborative; RoL; Molecular; patterns; redox

The bacterial diseases of plants lead to substantial crop damage and significant economic losses each year. Plants activate defense responses locally (i.e., at the infection site) and systemically when confronted with pathogenic bacterial infections. The goal of this project is to better understand these plant defense response pathways and to better understand how plants defend against bacterial infections in general. This project will be used to provide research experiences to students from Historically Black Colleges and Universities in MS, NC, and GA. These partnerships' critical goal will be to generate sustainable long-term collaborations with faculty, train undergraduate students in STEM research, and build a pipeline of underserved students for related career tracks. Online symposia, video lectures and practical workshops will be organized on project-related themes including plant synthetic biology. Knowledge of the molecular logic and organization of redox signaling networks is necessary to enhance our understanding of the adaptive system that governs responses to environmental challenges in eukaryotes. A multi-disciplinary team with complementary expertise will engage in innovative experimental and computational approaches to elucidate redox modulated signaling pathways that underpin synchronous and pulsatile gene activity in plant systemic immunity. The project consists of three components. First, high-throughput transcription profiling will identify recurrent spatial and temporal patterns in the plant immune transcriptome. Second, advanced redox proteomics will identify oxidative modifications of protein cysteines with a focus on regulatory thiols. Finally, a theoretical and modeling framework will be generated to conceptualize and analyze the significance of signal periodicity in plant systemic immunity. Successful implementation of the project will impact our understanding of redox processes in information encoding in biological systems and generate methods and algorithms widely applicable to study redox modifications of proteins and oscillatory dynamics of biological systems.This project is co-funded by the Systems and Synthetic Biology cluster in the Division of Molecular and Cellular Biosciences and the Plant-Biotic Interactions Program in the Division of Integrative Organismal Systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物细菌性病害每年都给农作物造成严重的危害和巨大的经济损失。植物在局部激活防御反应（即，在感染部位）和当面临病原性细菌感染时全身性地感染。 该项目的目标是更好地了解这些植物防御反应途径，并更好地了解植物如何防御细菌感染。 该项目将被用来提供研究经验，从历史上的黑人学院和大学在MS，NC和GA的学生。这些合作伙伴关系的关键目标将是与教师建立可持续的长期合作关系，培养STEM研究的本科生，并为相关职业轨道建立一个服务不足的学生管道。将组织关于项目相关主题的在线研讨会、视频讲座和实践研讨会，包括植物合成生物学。氧化还原信号网络的分子逻辑和组织的知识是必要的，以提高我们对真核生物的适应性系统，管理对环境挑战的反应的理解。 一个具有互补专业知识的多学科团队将参与创新的实验和计算方法，以阐明氧化还原调节的信号通路，这些信号通路支持植物系统免疫中的同步和脉冲基因活性。该项目由三个部分组成。首先，高通量转录谱分析将识别植物免疫转录组中的重复性空间和时间模式。其次，先进的氧化还原蛋白质组学将确定蛋白质半胱氨酸的氧化修饰，重点是调节硫醇。最后，将产生一个理论和建模框架，概念化和分析信号周期性在植物系统免疫中的意义。该项目的成功实施将影响我们对生物系统信息编码中氧化还原过程的理解，并产生广泛适用于研究蛋白质氧化还原修饰和生物系统振荡动力学的方法和算法。该项目由分子和细胞生物科学部系统与合成生物学集群和植物-综合有机体系统部的生物相互作用计划。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。