Collaborative Research: Function and Regulation of Thimet Oligopeptidase-Mediated Proteolytic Pathways in Plant Stress

合作研究：蒂美特寡肽酶介导的蛋白水解途径在植物胁迫中的功能和调节

• 批准号: 1714405
• 负责人: Leslie Hicks
• 金额: $ 35.23万
• 依托单位: University of North Carolina at Chapel Hill
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1714405&HistoricalAwards=false
• 关键词: Collaborative; Research; Function; Regulation; Thimet

Plants are dynamic living systems in which external and internal signals are integrated to facilitate and sustain growth and health. This research is targeted toward understanding the understudied phenomena of how plants use protein cleavage and degradation in response to stress signals. This knowledge may lead to increased survival rates of crops, and a further understanding of the adaptation and tolerance mechanisms utilized by these crops. A diverse population of graduate, undergraduate and high school students will be trained in modern methods of biochemistry, analytical chemistry, and systems and computational biology. Undergraduates will have the opportunity to participate in a summer exchange Research Experience for Undergraduates (REU) program between Mississippi State University and University of North Carolina at Chapel Hill and the PIs will develop an exhibit with scientific demonstrations for the NC Science Festival targeted to elementary and middle school students. The project employs the plant Arabidopsis as a model to elucidate the contributions of the proteolytic pathways mediated by TOP1 and TOP2 thimet oligopeptidases through integrated biochemical, proteomics, and systems biology approaches. Unique methods have been utilized in the preliminary studies to characterize the interaction of thimet oligopeptidases with the plant stress hormone salicylic acid. Genetic and biochemical experiments uncovered roles for TOPs in pathogen defense as possible "redox sensors". Fundamental differences between TOP1 and TOP2 cellular functions have been revealed experimentally and captured in a cell-level computational model. The research will expand on these findings by addressing the following aspects: (i) characterize redox-specific modifications mediated by and regulating TOP1 and TOP2, (ii) identify the substrate repertoire of TOP1 and TOP2, and (iii) expand and verify predictive models bridging molecular and structural characteristics of TOP1 and TOP2 with the whole-cell response. Upon the successful completion of the proposed research, a framework for TOP signaling in plants will be established and provide critical insights into how the interplay between proteolytic pathways and defense hormone signaling can be harnessed for the benefit of adaptation and tolerance mechanisms of crops.This project is co-funded by the Systems and Synthetic Biology Program in the Division of Molecular and Cellular Biosciences and by the NSF EPSCoR Program.

植物是动态的生命系统，在这个系统中，外部和内部的信号被整合起来，以促进和维持生长和健康。这项研究旨在了解植物如何利用蛋白质裂解和降解来响应胁迫信号的未被充分研究的现象。这一知识可能会提高作物的存活率，并进一步了解这些作物所利用的适应和耐受机制。不同的研究生、本科生和高中生将接受现代生物化学、分析化学、系统和计算生物学方法的培训。本科生将有机会参加密西西比州立大学和北卡罗来纳大学教堂山分校之间的暑期本科生研究经验交流(REU)项目，PI将为面向中小学生的NC科学节开发一个科学演示展览。该项目以植物拟南芥为模型，通过综合生化、蛋白质组学和系统生物学的方法，阐明了TOP1和TOP2硫代寡肽酶介导的蛋白降解途径的贡献。在初步研究中，已经使用了独特的方法来表征甲硫氨酸寡肽酶与植物胁迫激素水杨酸的相互作用。遗传和生化实验揭示了TOPS在病原体防御中的作用，可能是“氧化还原感受器”。TOP1和TOP2细胞功能之间的根本差异已经从实验上揭示出来，并在细胞水平的计算模型中捕捉到。这项研究将通过以下几个方面来扩展这些发现：(I)表征TOP1和TOP2介导和调节的氧化还原特异性修饰，(Ii)确定TOP1和TOP2的底物谱系，以及(Iii)扩展和验证将TOP1和TOP2的分子和结构特征与全细胞反应联系起来的预测模型。在这项拟议的研究成功完成后，植物中TOP信号的框架将被建立，并为如何利用蛋白质分解途径和防御激素信号之间的相互作用来促进作物的适应和耐受机制提供关键的见解。该项目由分子和细胞生物科学部门的系统和合成生物学计划以及NSF EPSCoR计划共同资助。

项目成果

Proteome-Wide Analysis of Cysteine Reactivity during Effector-Triggered Immunity.

效应器触发免疫过程中半胱氨酸反应性的全蛋白质组分析。

• DOI: 10.1104/pp.18.01194
• 发表时间: 2019
• 期刊: Plant physiology
• 影响因子: 7.4
• 作者: McConnell,EvanW;Berg,Philip;Westlake,TimothyJ;Wilson,KatherineM;Popescu,GeorgeV;Hicks,LeslieM;Popescu,SorinaC
• 通讯作者: Popescu,SorinaC

Evaluation of linear models and missing value imputation for the analysis of peptide-centric proteomics

• DOI: 10.1186/s12859-019-2619-6
• 发表时间: 2019-03-01
• 期刊: BMC BIOINFORMATICS
• 影响因子: 3
• 作者: Berg, Philip;McConnell, Evan W.;Popescu, George V.
• 通讯作者: Popescu, George V.