MRI-Consortium: Acquisition of a Shipboard Sorting High Speed Flow Cytometer for Research and Education

智力价值PI正在申请资金，以购买船用分选流式细胞仪设施，包括船用货车、MoFlo XDP细胞仪、气相色谱仪和附件，以及带有荧光显微镜稳定平台的倒置显微镜。该设施将用于：确定和分离新的海洋微生物以供培养和实验；在物种和单细胞一级确定和测量海洋微生物的各种代谢活动；并利用细胞分选器作为微型采样器开发新的方法和应用。该设施将有助于开展一系列工作的研究，包括：生物成因硫化合物的生物地球化学；不同地点分类海洋浮游植物的细胞内DMSP水平；分类海洋藻类种群的显微鉴定；以及分类海洋有害藻华物种的毒素浓度。更广泛的影响拟议中的收购将有助于增强霍林斯海洋实验室的研究能力和合作努力，该机构代表着五个实体之间的合作伙伴关系，包括查尔斯顿学院、南卡罗来纳州自然资源部、南卡罗来纳州医科大学、国家标准与技术研究所和美国国家海洋和大气局S海岸环境健康和生物分子研究中心。此外，该提案指出，研究生研究将涉及使用这种拟议的仪器来测试涉及分子探测器的新方法的论文项目。本科生还将有机会接受培训并使用拟议的工具，作为现有REU计划的一部分，包括一个专注于代表性不足的群体的计划。

Intellectual MeritThe PI is requesting funds to acquire a shipboard sorting flow cytometer facility including a shipboard van, MoFlo XDP cytometer, gas chromatograph and accessories, and inverted microscope with stabilizing platform for epifluorescence microscopy. The facility would be used to: identify and isolate new oceanic microbes for cultivation and experimentation; identify and measure various metabolic activities of microorganisms in the ocean at the species and single cell level; and develop new approaches and applications using a cell sorter as a micro-sampler. The facility would enable research on a suite of efforts including: biogeochemistry of biogenic sulfur compounds; intracellular DMSP levels in sorted marine phytoplankton in a variety of locations; microscopic identification of sorted marine algal populations; and toxin concentrations in sorted marine harmful algal bloom (HAB) species. Broader ImpactsThe proposed acquisition will serve to enhance the research capabilities and collaborative efforts at Hollings Marine Laboratory, an institution that represents a partnership between five entities including the College of Charleston, the South Carolina Division of Natural Resources, the Medical University of South Carolina, the National Institute for Standards and Technology, and NOAA?s Center for Coastal Environmental Health and Biomolecular Research. Additionally, the proposal states that graduate research will involve thesis projects using this proposed instrumentation to test new methods involving molecular probes. Undergraduates will also have the opportunity to be trained on and use the proposed instrumentation as part of existing REU programs, including one focused on underrepresented groups.