SBIR Phase II: Development of Design and Operational Criteria of Continuous Culture Hatchery Techniques for the Production of Brachionus rotundiformis (s-type) rotifers

这个小型企业创新研究（SBIR）二期项目的总体目标是将轮虫（轮状Brachionus rotundiformis； s型）的稳健、连续养殖生产系统商业化，轮虫是海洋幼鱼的主要活饲料来源。美国海洋水产养殖业发展的一个主要瓶颈仍然是缺乏商业活饲料生产技术来支持成长型产业。特别是对于轮虫的培养，目前存在有限的标准化设计和操作标准的连续培养方法，可以促进提高生产力和供应的一致性。无法经济有效地（数量上）和持续地（数量和质量上）供应微藻/浮游动物饲料仍然是海洋水产养殖业扩大的一个主要限制。这项技术的更广泛影响是简化鱼种生产，这将导致海洋孵化场的迅速扩大，从而大大增加在池塘、海洋网箱和/或室内循环系统中养殖的低成本鱼苗和鱼种的供应。这项研究有可能通过消除增加许多海洋物种产量的一个关键限制因素，即鱼苗和鱼种的缺乏和高成本，帮助启动海洋水产养殖业。更受欢迎的海洋鱼类的水产养殖生产将减轻目前受到严重威胁或过度捕捞的商业鱼类种群的压力，并允许使用商业配方饲料在高度控制和生物安全的条件下进行养殖。

This Small Business Innovative Research (SBIR) Phase II project's overall goal is to commercialize a robust, continuous culture production system for rotifers (Brachionus rotundiformis; s-type), the major live feed source for marine larval fish. A major bottleneck in the expansion of the marine aquaculture industry in the United States remains the lack of commercial live feed production technologies to support the grow-out industry. Specifically for rotifer culture, there exist limited standardized design and operational criteria for continuous culture methods that could facilitate increased productivity and consistency of supply. The inability to supply microalgal/zooplankton feeds cost-effectively (quantity) and consistently (quantity and quality) continues to be a major limitation to the expansion of the marine aquaculture industry.The broader impacts of this technology are simplification of fingerling production which will lead to rapid expansion of marine hatcheries and hence a dramatic increase in the availability of lower cost marine fry and fingerlings for aquaculture grow-out in ponds, ocean cages and/or indoor recirculating systems. This research has the potential to help jump-start the marine aquaculture industry by eliminating one of the key limiting factors to increasing the production of many marine species, i.e. the unavailability and the high cost of fry and fingerlings. Aquaculture production of the more popular marine finfish will relieve the pressure on commercial fish stocks that are currently severely threatened or overfished and allow culture under highly controlled, biosecure conditions using commercial formulated diets.