Pattern formation in self-organized surface confined peptides

智能优点：本提案的目的是阐明限定在界面上的定义良好的片状形成肽的自组装和图案形成的基本方面。我们的方法包括三个步骤。(1)建议设计和合成简单的周期肽序列，产生表面活性链，其自组织成聚集体以形成作为肽序列的函数的图案。合理的多肽设计使我们能够系统地探索疏水性、静电性和分子大小对图案形成的作用。(2)建议使用一套界面表征工具来直接解决组装现象。可以使用CD和ATR-FTIR检查多个长度尺度，包括使用CD和ATR-FTIR检查分子长度尺度，以及使用布鲁斯特角度显微镜、荧光显微镜和椭偏测量法在超分子长度尺度上进行检查。(3)建议使用二维状态方程来定义界面上新生聚集体的相行为和临界表面浓度。随后，我们可以应用这些参数来预测图案形成的尺寸。广泛影响：设计合理的多肽和多肽正迅速成为纳米结构材料中的有用成分，应用范围从药物输送到能量存储。如果成功，PI相信拟议的研究将产生对受限下生物分子组装的基本理解，这些想法可以直接应用于杂交材料设计。CCNY的使命是为高度多样化的学生群体提供广泛领域的高质量本科和研究生教育，其中34%是西班牙裔，24%是黑人，17%是亚裔，11%是白人，将我们学院归类为少数族裔服务机构。PI致力于这一任务，要求在不牺牲研究或教育质量的情况下，向不同背景的学生教授多种主题。除了研究生培训，本科和高中对这项工作的研究支持将通过两个机制进行协调。(1)NSF资助了CCNY的NUE、REU和PREM项目，为本科生和高中生提供研究经验。(2)在过去的三年里，PI领导了CCNY-HSMSE同伴学习计划。该项目为本科生和高中生提供了在课堂和实验室中从事工程和科学研究的机会。PIs已经成功地开发了这一机制，允许本科生和高中生在一个有吸引力的环境中探索基础工程和科学问题，在这种环境中，同行学习导致共同点，以增强学习体验。

0967365TuIntellectual Merit:The objective of this proposal is to elucidate the fundamental aspects of selfassembly and pattern formation of well defined sheet forming peptides confined at interfaces. Our approach involves three steps. (1) It is proposed to design and synthesize simple periodic peptide sequences, yielding surface active â-strands that self organize into aggregates to form patterns as a function of the peptide sequence. Rational peptide design allows us to systematically explore the role of hydrophobicity, electrostatics and molecular size on pattern formation. (2) It is proposed to directly address the assembly phenomena using a set of interfacial characterization tools. Multiple length scales can be examined, including molecular length scales, using CD and ATR-FTIR, and at supramolecular length scales, using brewster angle microscopy, fluorescence microscopy and ellipsometry. (3) It is proposed to use two dimensional equations of state that define both the phase behavior and the critical surface concentrations of nascent aggregates at the interface. Subsequently, we can apply these parameters to predict the dimensions of pattern formation.Broader Impact:Rationally designed peptides and polypeptides are rapidly becoming useful components in nanostructured materials for applications ranging from drug delivery to energy storage. If successful, the PI's believe that the proposed research will yield fundamental understanding of biomolecular assembly under confinement, and these ideas can be directly applied in hybrid materials design.CCNY's mission is to provide quality undergraduate and graduate education in a broad range of fields to a highly diverse student body, which is 34% Hispanic, 24% black, 17% Asian and 11% white, classifying our college as a Minority Serving Institution. The PI is dedicated to this mandate that asks for a multiplicity of subject matters to be taught to students of varying backgrounds without sacrificing quality of research or education. In addition to graduate training, undergraduate and high school research support for this work will be coordinated through two mechanisms. (1) NSF has funded NUE, REU and PREM programs at CCNY to provide research experiences for both undergraduate and high school students. (2) The PI has led the CCNY-HSMSE Peer-Learning Program for the last three years. This program provides an opportunity for undergraduates and high school students to engage in engineering and science in the classroom and lab. The PIs have successfully developed this mechanism that allows undergraduate and high school students to explore fundamental engineering and science questions in an engaging setting where peer learning results in common ground to enhance the learning experience.