Mechanistic Studies on Quinoline and Other Photoremovable Protecting Groups for Biological Applications

化学结构、动力学和机制项目支持乔治亚大学的Timothy M. Dore教授，他将探索光去保护的机制，并优化对双光子激发（2PE）敏感的光去除保护基团（PPG）的灵敏度。大量的光谱技术将用于研究喹啉和其他对2PE敏感的PPG。这些材料有潜力用于光调节生物效应的作用。优化其光化学性质以用于生物应用需要对光保护反应机理有很好的了解。这项研究将扩大现有光保护基团的基础，并进行光物理和光化学研究，旨在更好地了解系统运作的机制。本提案中的研究将使新工具和技术的发展能够应用于神经科学，发育生物学，生物化学，生理学和药物化学的基础科学问题。在美国国家科学基金会化学部化学结构、动力学和机制项目的支持下，Timothy M. Dore博士将制备合适的化合物，并了解其光物理特性，这将使它们在双光子激发中对生物过程的精确三维控制有用。除了工作对生物科学的关系产生更广泛的影响外，包括本科生研究人员是该提案的主要优势。本研究的多学科性质为研究与教育相结合提供了一个很好的媒介，培养学生成为对有机化学和光化学有深入了解的优秀学者。本科生，包括那些来自代表性不足群体的学生，在研究中发挥了重要作用。该项目已培养了超过25名本科生，其中许多人是论文和手稿的合著者，并已进入博士课程。这项研究计划包括让代表性不足的群体参与科学研究。

The Chemical Structure, Dynamics and Mechanisms Program supports Professor Timothy M. Dore at the University of Georgia who will explore the mechanism of photodeprotection and optimize the sensitivity of photoremovable protecting groups (PPG) that are sensitive to two-photon excitation (2PE). A host of spectroscopic techniques will be used to study quinoline and other PPG with sensitivity to 2PE. These materials have the potential to be used to photoregulate the action of biological effectors. Optimizing their photochemical properties for biological applications requires a good understanding of the photodeprotection reaction mechanism. The study will expand the base of photoprotecting groups available, as well as carry out photophysical and photochemical studies aimed at providing an improved understanding of the mechanism by which the systems operate. The research in this proposal will enable the development of novel tools and techniques to apply to fundamental scientific questions in neuroscience, developmental biology, biochemistry, physiology, and medicinal chemistry.With the support of the Chemical Structure, Dynamics and Mechanisms Program in the Chemistry Division at the National Science Foundation, Dr. Timothy M. Dore will prepare appropriate chemical compounds and understand the photophysical characteristics that will make them useful in two-photon excitation for precise 3-D control of biological processes. In addition to the broader impact of the relationship of the work to the biological sciences, the inclusion of undergraduate researchers is a major strength of the proposal. The multidisciplinary nature of this research provides an excellent medium to integrate research and education and to train students to become outstanding scholars with a depth of understanding of organic chemistry and photochemistry. Undergraduate students, including those from underrepresented groups, play a major role in the research. The PI has trained over 25 undergraduates and many are coauthors on articles and manuscripts and have matriculated into doctoral programs. The research plan includes the participation of underrepresented groups in science.