IDR: Rapid, three-dimensional microscopy of cell-cell interactions in suspension

这个项目将开发一种独特的最先进的技术，以获得一种极其重要的细胞--免疫细胞--的功能和结构的快速三维图谱。它将利用悬浮细胞在其最自然的环境中(即自由悬浮而不存在底物)的高空间和时间分辨率的快速光学成像。这一新仪器将允许人们在毫秒级的时间尺度上测量和记录细胞在3个维度上的生理变化。该系统将在亚细胞水平上实时定量评估细胞组织和重组，从而在细胞成像、细胞生物学、免疫学、病毒学等多个科学学科中取得重大进展。这一新颖的仪器将显著增强在分子水平上量化悬浮免疫细胞的快速细胞过程的能力，这是目前的技术所不可能做到的。它将使人们能够操纵和增加细胞相互作用的数量，并动态跟踪它们，从而在很大程度上控制细胞相互作用的研究。

1014838HuserThis project will develop a unique state-of-the-art technology to obtain rapid three-dimensional maps of the function and structure of an extremely important class of cells: immune cells. It will utilize rapid optical imaging at high spatial and temporal resolution of suspension cells in their most natural environment (i.e. freely suspended without substrates present). This new instrument will allow one to measure and record physiological changes in cells in 3 dimensions at millisecond time scales. This system will lead to significant advancement in multiple scientific disciplines, i.e. cellular imaging, cell biology, immunology, virology, etc. by enabling the quantitative assessment of cellular organization and reorganization due to changes in their environment at the sub-cellular level in real-time. This novel instrumentation will significantly enhance the ability to quantify rapid cellular processes of suspended immune cells at the molecular level, something that is not possible with current technology. It will enable the study cellular interactions with a great degree of control by enabling one to manipulate and increase the number of cellular interactions and follow them dynamically.