SBIR Phase I: Development of a Commercial Culture of Dehalococcoides for Anaerobic Treatment of PCB's

这个小型企业创新研究(SBIR)阶段的项目致力于开发非细菌培养来降解污染土壤和沉积物中的多氯联苯(PCB)。多氯联苯是一种持久性的有毒污染物，它积聚在动物的脂肪组织中，对鱼类和人类造成污染。多氯联苯降解细菌一直难以生产。2009年，一种容易生长的细菌种类显示，通过生物修复C NC(BCI)来降解最有害和致癌类型的多氯联苯。这一发现允许开发一种厌氧降解多氯联苯的培养物，用于经济高效地接种到多氯联苯污染的沉积物中。这项拟议的研究将证明BCI的培养物广泛脱氯三种商业多氯联苯混合物的能力，并将开发快速培养方法。这项研究开发的更广泛/商业范围的研究将降解土壤和沉积物中的多氯联苯。多氯联苯是一种有毒化学物质，历史上在许多工业应用中作为耐热流体使用，包括电力工业中的变压器。制造和处置了数亿磅，导致土壤和河流沉积物受到污染。BCI的最新发现是，一种脱氯菌能够脱氯多氯联苯。为BCI提供了一个为污染场地接种多氯联苯降解菌的商业培养的机会。该产品的营销将得到我们已经成功的用于处理各种氯化合物污染的其他脱氯培养的补充。公用事业行业的主要利益相关者和补救承包商，已经在与BCI合作和/或获得培养中表现出来。

This Small BusinessI nnovationR esearch( SBIR) Phase1 project addressesth e developmento f abacterial culture to degrade PCBs (polychlorinated biphenyls) in contaminated soils andsediments. PCBs are persistent, toxic contaminants which accumulate in the fatty tissues ofanimals,c ontaminatingf ish and humans.P CB-degradingb acteriah ave beend ifficult to produce.In2009, a readily-grownb acterials peciesw as shownb y BioremediationC onsultingI nc (BCI) todegrade the most harmful and carcinogenic type of PCBs. This finding allows the developmentof an anaerobic PCB-degrading culture for cost-etfective inoculation into PCB-contaminatedsediments. The proposed research will document the ability of BCI's culture to extensivelydechlorinate three types of commercial PCB mixtures, and will develop methods of rapidculturing.The broader/commerciailm pactso f this researcha ret he developmento f a bacteriai noculantt hatwill degrade PCBs in soil and sediment. PCBs are toxic chemicals used historically as heatresistantfluids in many industrial applications, including transformers in the electric utilityindustry. Several hundred million pounds were manufactured and disposed, resulting incontamination of soils and river sediments. The recent finding at BCI, that a strain ofdechlorinating bacteria is capable of dechlorinating PCBs. provides an opportunity for BCI todevelop a commercial culture for inoculating contaminated sites with PCB-degrading bacteria.The marketing of this product will be complementedb y our already-successfusl aleso f otherdechlorinating cultures used in treatment of contamination by diverse chlorinated compounds.Major stakeholdersin the utility industries,a nd remediationc ontractors,h ave expressedin terestin partnering with BCI and/or obtaining culture.