SBIR Phase II: Commercialization and Scale Up of Ultra Low Fouling and Functionalizable Surface Coatings Based on Zwitterionic Polycarboxybetaine

这个小型企业创新研究(SBIR)第二阶段项目旨在展示两性离子技术在蛋白质相互作用分析中的非凡表现。两性离子聚羧酸甜菜碱(PCB)不仅对非特异性蛋白质的吸附具有很强的抵抗力，而且具有丰富的官能团，可以方便有效地通过常规化学方法固定生物大分子。这种双功能特性使印刷电路板有别于其他现有的低污染材料，并能够在复杂介质中进行诊断或分子识别。在这个项目中，高质量的两性离子材料和涂层将以低成本和简单的生产工艺放大。预期的结果是，人们可以将分子识别元件直接固定到无污染的背景上，以用于广泛的应用。该项目的更广泛的商业影响将是为生物医学和工程应用提供一种有效的方法来保护表面免受不必要的相互作用。两性离子技术具有成本低、稳定性好、效果好和附加功能等优点。对于蛋白质相互作用分析和医疗诊断，这项技术将提高生物传感器的灵敏度和特异度，并能够检测未稀释的人血浆和血清中的分析物。

This Small Business Innovation Research (SBIR) Phase II project aims to demonstrate the extraordinary performance of zwitterionic technology in protein interaction analysis. Zwitterionic polycarboxybetaine (pCB) is not only highly resistant to nonspecific protein adsorption, but also has abundant functional groups for the convenient and effective immobilization of biomolecules via conventional chemistry. This dual-functional property distinguishes pCB from other existing low-fouling materials, and enables diagnostics or molecular recognition in complex media. In this project, high-quality zwitterionic materials and coatings will be scaled up with low-cost and simple production process. The expected outcome is that one can immobilize molecular recognition elements directly onto a non-fouling background for a wide range of applications.The broader/commercial impacts of this project will be the potential to offer an effective approach in protection of surfaces from unwanted interactions in complex media for biomedical and engineering applications. Zwitterionic technology has superior advantages of low cost, stability, effectiveness and additional functionalities. For protein interaction analysis and medical diagnostics, this technology will improve the sensitivity and specificity of a biosensor, and enable the detection of analytes in undiluted human blood plasma and serum.