Novel Pt(II) Reagents for RNA Biochemistry

通过这个奖项，生命过程化学项目资助俄勒冈大学的Victoria DeRose教授与Michael Haley教授合作研究，开发和使用Pt（II）试剂用于RNA交联，以帮助确定RNA的三级结构。研究人员将采用过渡金属络合物合成、RNA内聚研究和更全面的化学定向RNA标记的组合。后内收的要素，第二阶段的标签也设想。化学、分子生物学和光谱技术的结合将用于梳理通过化学生物学方法生成的标记rna的三级结构信息。如果成功，这种方法可能会成长为一种更广泛适用于核酸结构生物学领域的化学生物学工具，具有潜在的重要科学影响。该项目还为学生提供了共同指导的机会，由一名合成无机/有机化学家和一名在金属-核酸相互作用和光谱学方面有良好记录的RNA化学生物学家指导。更广泛的教育影响还包括显著的本科研究组成部分，以及继续努力使妇女和传统上代表性不足的群体成员接触现代研究科学。

With this award, the Chemistry of Life Processes Program is funding Professor Victoria DeRose of the University of Oregon for collaborative studies with Professor Michael Haley to develop and use Pt(II) reagents for RNA-crosslinking to aid in the determination of RNA tertiary structure. The researchers will employ a combination of transition metal complex synthesis, RNA adduction studies, and more global, chemically directed RNA-tagging. Elements of post-adduction, second-stage tagging are also envisioned. A combination of chemical, molecular biological and spectroscopic techniques will be used to tease out tertiary structural information from the tagged RNAs generated through this chemical biology approach. If successful, this approach may grow into a more broadly applicable chemical biology tool for the field of nucleic acid structural biology, a potentially important scientific broader impact. The project also offers the opportunity for co-mentorship of students, under both a synthetic inorganic/organic chemist and an RNA chemical biologist with a strong record in metal-nucleic acid interactions and spectroscopy. The educational broader impacts also comprise a notable undergraduate research component and continued efforts to expose women and members of traditionally underrepresented groups to modern research science.