Detection and amplification of DNA at near room temperature

总体目标是研究使用称为悬臂梁传感器的设备检测和改进DNA。我们将系统地研究强表面振动对结合双链DNA行为的作用。 我们假设强烈的振动会打开分子。 目前，我们通过加热来进行这样的反应。 我们的初步工作表明，振动定向应用将导致DNA？It’这两部分要分开。 我们将研究是否可以实现增加传感器上的DNA的方法。 如果这两个目标得以实现，我们就有可能检测到极少量的DNA。 这种能力意味着我们可以快速检测有害细菌，拯救生命;快速而廉价地检测人类和动物疾病。知识上的优点：三个优点是：（1）将开发一种新的和更便宜的方法来检测DNA在一个非常低的水平。(2)（3）培养在核酸化学（动力学和热力学）、机电谐振器和测量科学等广泛领域交叉的跨学科领域的博士生。更广泛的影响：各种背景的学生？高中生，各学科的本科生？将接触到DNA测量技术 一个重要的广泛影响将是一种新的方法，其灵敏度类似于或优于目前用于疾病诊断和有害细菌存在的昂贵方法。

The overall goal is to investigate both detection and improvement of DNA using a device known as cantilever sensor. We will investigate systematically the role of intense surface vibration on bound double stranded DNA behavior. We hypothesize that the intense vibration will open up the molecule. Currently we do such a reaction by heating. Our preliminary work suggests that vibration directionally applied will cause DNA?s two parts to come apart. We will investigate if a method of increasing the DNA on the sensor can be accomplished. If these two goals are accomplished we may be able to detect DNA that is present in extremely small amounts. The implication of such a capability is we can detect harmful bacteria quickly saving lives; detect human and animal diseases rapidly and inexpensively. Intellectual Merits: Three merits are: (1) A new and cheaper approach will be developed for the detection of DNA at a very low level. (2) We will also develop a fundamental understanding of how vibration intensity affects DNA reaction, (3) Training of PhD students in the interdisciplinary area at the intersection of the broad field of nucleic acid chemistry (kinetics and thermodynamics), electromechanical resonators, and measurement science. Broader Impacts: Students of various backgrounds ? high school, undergraduates in various disciplines ? will be exposed to DNA measurement techniques. An important broad impact will be a new methodology that has sensitivity similar or better than the current expensive methods used in disease diagnosis and presence of harmful bacteria.