High resolution Imaging Mass Spectrometry (IMS) of neuropeptides in dynamic systems

该项目的目的是开发一个MSI平台，用于高分辨率分析中枢神经系统中结构和功能最可变的信使分子，即神经肽的空间定位和动力学。虽然神经肽学的新领域允许对样本中的神经肽进行表征，但由于缺乏测量工具，人们对其空间动力学知之甚少。迫切需要改进基于质谱学的成像/定位。在神经肽MSI中，保持足够的分辨率和高灵敏度是神经肽MSI的挑战。申请者包括在生物化学、神经科学和遗传学方面的长期经验。最重要的是，在该提案的背景下，他们在MALDI-TOF质谱学和多肽学方面有很强的背景，并专注于开发分析已识别和解剖的神经元、果蝇和其他昆虫神经和外围组织中的神经肽的方法或微星系统本身的方法(Sweedler)。该提案分为两个子项目：1)为分析哺乳动物多肽能系统内的即时补偿机制而开发MSI。2)MSI--社会性昆虫发育和行为中神经肽动态的功能分析方法的发展。

The purpose of the project is the development of a MSI-platform for high resolution analysis of spatial localization and dynamics of the structurally and functionally most variable messenger molecules in the CNS, namely neuropeptides. While the new field of neuropeptidomics allows the neuropeptides from a sample to be characterized, their spatial dynamics are poorly understood because of a lack of measurement tools. There is a critical need for improved mass-spectrometrybased imaging/localization. The maintenance of a sufficient resolution in combination with high sensitivity as typical of conventional direct tissue profiling is a challenge in MSI of neuropeptides. The applicants cover longstanding experience in Biochemistry, Neuroscience, and Genetics. M most importantly in the context of the proposal, they have a strong background in MALDI-TOF mass spectrometry and peptidomics, and focused on the development of methods for the analysis of neuropeptides in identified and dissected neurons, in Drosophila and other insect nervous and peripheral tissue or method development in MSI itself (Sweedler). The proposal is divided in two subprojects: 1) Development of MSI for the analysis of immediate compensation mechanisms within the mammalian peptidergic system. 2) Development of MSI-approaches for the functional analysis of neuropeptide dynamics in development and behaviour of a social insect.