Genomic Architecture Underlying Behavioral Isolation and Speciation

不同的配偶识别特征可以在物种形成中发挥关键作用，通过在分化种群中进化的相似交配，减少新形成物种之间的基因流动。雄性和雌性之间交流的变化预示着雄性交配信号和雌性偏好之间的遗传相关性。测试关于种内通信系统的雄性和雌性遗传成分的假设将促进对这些关联如何产生的了解。利用新一代测序方法，包含与物种起源有关的分化基因的遗传系将被绘制到基因组中，并对其基因产物进行测序。所采取的方法是1)测试鸣叫和偏好基因的共定位，2)将结果翻译成核苷酸基因组图，以产生潜在行为变化的候选基因，3)建立与不同行为相关的表达基因数据集。还将进行有针对性的基因组组装和基因注释。数据将根据数据类型存储和可用。WAV文件将存储在DYRAD （www.dyrad.com）上，而序列数据将存储在NCBI序列读取档案中。该项目将产生多重更广泛的影响。许多说明行为生态学和进化原理的引人入胜的行为来自“非模式”生物，而对行为的遗传见解往往来自“模式”遗传生物。将自然行为变异（雄性信号和雌性偏好）与先进的基因组方法相结合，为模型遗传学研究和自然行为系统之间提供了一座桥梁。洞见有望成为解释多元化的最佳实证和概念桥梁之一。从教育和推广的角度来看，该项目将为本科生和研究生提供培训机会，并将通过向公共科学中心和以昆虫为中心的博物馆活动捐款，继续努力与公众接触。

Divergent mate recognition traits can play a pivotal role in speciation by curtailing gene flow between newly formed species through the evolution of like-mating within differentiated populations. Changes in communication between males and females predict a genetic correlation between male mating signals and female preferences. Testing hypotheses about the male and female genetic components of the intraspecific communication system will advance knowledge of how these associations arise. Using next-generation sequencing approaches, genetic lines that contain differentiated genes involved the origin of species will be mapped in the genome and their gene products will sequenced. The approach taken is to 1) to test the co-localization of song and preference genes, 2) translate the results into a nucleotide genome map to generate candidate genes underlying behavioral change, and 3) build a data set of expressed genes that associates with different behaviors. A targeted genome assembly and gene annotation will also be undertaken. Data will be stored and available based upon type of data. WAV files will be stored on DYRAD (www.dyrad.com), while sequence data will be deposited in the NCBI Sequence Read Archive. The project will have multiple broader impacts. Many of the fascinating behaviors that illustrate principles in behavioral ecology and evolution come from "non-model" organisms whereas the genetic insights into behavior often come from "model" genetic organisms. The integration of natural behavioral variation (male signal and female preference) with advanced genomic approaches provides a bridge between model-genetic study and natural behavioral systems. Insights promise one of the best empirical and conceptual bridges to explain diversification. From the education and outreach perspective, the project will provide training opportunities for undergraduate and graduate students, and efforts to reach out to the public will continue via contributions to public science centers and museum events centered on insects.