Enabling Highly Sensitive and Specific Detection from Undiluted Blood Using Paper Sensors

护理点设备需要简单而廉价的平台，具有对复杂介质的高灵敏度。纸张是最便宜的检测开发平台，通常可以比传统传感器更快地获得结果。目前改进纸质检测技术的努力依赖于物理吸附的配体（配体数量和活性有限），依赖于阻断剂来钝化非特异性蛋白质吸附（检测灵敏度有限，仅适用于简单介质或稀释的样品），并且由于样品的快速蒸发（通常在检测区域接收不到原始样品的50%）而导致样品保留率低。两性离子聚羧基甜菜碱（PCB）表面涂层能够在未稀释的人血浆和血清中实现高灵敏度和特异性检测。这项工作的目的是实现廉价，但高灵敏度的纸基检测从复杂的介质使用两性离子化学。

Point-of-care devices require simple yet inexpensive platforms with highsensitivity from complex media. Paper is the least expensive platform for assaydevelopment and often allows results to be obtained faster than conventional sensors.Current efforts to improve paper-based detection technologies rely on physicallyadsorbed ligands (with limited ligand amount and activity), rely on blocking agents topassivate nonspecific protein adsorption (with limiting detection sensitivity and suitableonly for simple media or diluted samples), and suffers from low sample retention due tothe rapid evaporation of samples (typically receiving less than 50% of the originalsample in the detection zone). Zwitterionic poly(carboxybetaine) (PCB) surface coatingshave been able to achieve highly sensitive and specific detection from undiluted humanplasma and serum. The objective of this work is to realize inexpensive, yet highlysensitive paper-based detection from complex media using zwitterionic chemistry.