A Biosensor Based on Magnetic Resonance Relaxation

1264721-传统培养和药敏试验继续成为金标准？用于在临床和其他环境中识别病原体。许多新的病原体检测方法旨在通过提供更快速、更准确和更便宜的检测来取代这一标准。非常有前景的技术，如聚合酶链式反应、表面增强拉曼和其他技术，已被证明擅长病原体检测，但最终用户还没有意识到他们的费用、广泛的样品制备和执行它们所需的技能集。该领域继续寻找一种快速、灵敏、特异、成本效益高且易于使用的检测技术。这项工作旨在开发一种基于磁共振弛豫开关的生物传感器。该方法利用了大量涉及核磁共振成像中使用的纳米造影剂的工作。这里的目的是开发一种检测方法，模拟人类对入侵病原体的免疫反应，释放109至1012个特定抗原，以确保与病原体的快速接触。这项拟议的技术将使用磁性纳米颗粒造影剂与特定的捕捉剂结合，以实现类似的接触目标。检测该物种将涉及监测溶液中水质子的平均弛豫时间(T2)，该时间对存在的磁性纳米颗粒的浓度和分布高度敏感。当多个纳米粒子附着在每个单独的靶物种上时，它们的分布将发生改变，相应地，平均质子弛豫时间也将发生变化。虽然这种方法利用了公认的核磁共振成像原理，但这种测量可以用一个简单的手持弛豫计来完成。S外行的说法这项建议将开发新的方法来检测食物或体液中的细菌和其他病原体。这项技术将使用磁性颗粒，其磁性将在细菌结合时以可预测的方式改变。作者设想能够使用一组独特的磁性纳米颗粒来检测多种细菌。

1264721 - ProrokConventional culture and susceptibility tests continue to remain the ?gold standard? for identifying pathogens in clinical and other settings. Many new pathogen detection approaches have aimed at replacing this standard by providing more rapid, more accurate and less expensive detection. Very promising techniques such as PCR, surface enhanced Raman, and others have proven adept at pathogen detection, but end users have not warmed up to their expense, extensive sample preparation and skill set necessary to perform them. The field continues to search for a rapid, sensitive, and specific detection technology that is cost-effective and easy to use. The proposed work aims to develop such a biosensor based on magnetic resonance relaxation switching. The method leverages a large body of work involving nanoscale contrast agents employed in nuclear magnetic resonance (NMR) imaging. The aim here is to develop a detection approach that mimics the human immune response to an invading pathogen, the release of 109 to 1012 specific antigens to guarantee quick contact with the pathogen. This proposed technique will employ magnetic nanoparticle contrast agents conjugated with specific capture agents to achieve a similar contact goal. Detection of the species will involve monitoring the average relaxation time (T2) of water protons in the solution, which is highly sensitive to the concentration and distribution of the magnetic nanoparticles present. With multiple nanoparticles attaching to each individual target species their distribution will be altered, and correspondingly, the average proton relaxation time will change. Although, this method leverages well established principles of NMR imaging, this measurement can be accomplished with a simple hand-held relaxometer. Content in layman?s termsThis proposal will develop new ways to detect bacteria and other pathogens in food or bodily fluids. This technology will use magnetic particles whose magnetic properties will change in predictable ways upon binding of bacteria. The authors envision being able to detect multiple bacteria using sets of unique magnetic nanoparticles.