Ring-Opening Metathesis Polymerization (ROMP) Coupled Lateral Flow Analysis for Portable BioSensing

PI已经开始开发一种耐人寻味的PCR扩增替代品，它可能会更简单，更具成本效益。PI之前在自由基“聚合放大”方法上的工作表明，它可以检测到少于2000个拷贝的DNA，假阳性和假阴性率可与PCR相媲美。这项建议试图通过使用开环歧化聚合(ROMP)来扩展这一新方法，作为避免氧与自由基聚合所起作用的一种方式，并将ROMP与侧向流动分析相结合作为新的可现场生物传感器的基础。这项拟议的研究具有变革性，有可能成为现场测试的基础，并在几个生物医学传感领域产生影响。这些结果将用于提高使用ROMP放大目标分析物存在的效率，并可作为例如巨细胞病毒(CMV)的第一线筛查。在各级培训任职人数不足的少数群体方面，该协会有着良好的记录，这项拟议的工作将加强这一外联工作。在之前的职业生涯中，她的工作效率相当高，发表了16篇文章，发表了21次会议演讲。

The PI has begun the development of an intriguing amplification alternative to PCR that could potentially be much simpler and more cost-effective. The PI's previous work on a free-radical "amplification?by-polymerization" approach demonstrated that it was possible to detect fewer than 2000 copies of DNA, with false positive and false negative rates comparable to PCR. This proposal seeks to extend this novel approach by using Ring-Opening Metathesis Polymerization (ROMP), as a way to avoid the problems oxygen plays with free-radical polymerization and to combine ROMP with lateral flow assay as the basis of a new fieldable biosensor. The proposed research is transformative and has the potential and has the potential to become the basis of a field assay and have an impact in several biomedical sensing areas. These results will be used to improve the efficiency of using ROMP for amplifying the presence of target analytes and could serve as a first-line screening for, e.g., cytomegalovirus (CMV). The PI has an excellent record for training underrepresented minorities at all levels and this proposed work will add to that outreach effort. Her productivity under the previous CAREER is quite good with 16 publications and 21 conference talks.