Multi-centre analysis of immune-regulatory mechanisms that protect from childhood asthma in distinct rural environments

这个多中心项目旨在解开环境不同地区儿童特应性哮喘的保护性免疫调节机制。独特的自然暴露于两种强烈保护儿童哮喘的环境中，即欧洲的农场和中国的农村地区，为评估来自欧洲和中国的四组特征良好的儿童的先天和适应性免疫系统，特别是树突状细胞和T调节细胞的相互作用提供了很好的机会：i)健康未暴露的儿童，ii)哮喘未暴露的儿童，iii)健康暴露的儿童，iv)哮喘暴露的儿童。我们将进一步评估“离体DC和Treg农业/农村特征”，将6岁儿童的分离血细胞暴露于来自欧洲和中国的含有哮喘保护微生物因子的环境样本中。因此，我们的目的是弄清楚这两种环境是否通过相同或不同的免疫途径发挥哮喘保护作用。该项目不仅将通过建立和交流专业知识和免疫学技术，为三国今后的过敏性哮喘免疫学研究提供深刻的基础。它也代表了一个无与伦比的机会来了解保护哮喘的免疫机制，这对未来预防策略的发展至关重要。

This multi-center project aims to disentangle protective immune regulatory mechanisms for atopic asthma in childhood in environmentally distinct areas. The unique natural exposure to two environments strongly protecting from childhood asthma, i.e. farms in Europe and rural areas in China offers the great opportunity to assess the interplay of the innate and adaptive immune system, specifically dendritic cells and T regulatory cells, in four groups of well-characterized children from both Europe and China: i) Healthy non-exposed children, ii) Asthmatic non-exposed children, iii) Healthy exposed, and iv) Asthmatic exposed children. We will further assess an “ex vivo DC and Treg farming/rural signature” by exposing isolated blood cells of 6 year old children to environmental samples containing asthma-protective microbial factors from Europe and China. Herewith, we aim to disentangle whether both environments exert asthma-protection via identical or different immunological pathways. This project will not only provide a profound basis for future immunological studies on allergic asthma in all three countries through establishment and exchange of expertise and immunological techniques. It also represents an unparalleled chance to understand the immune mechanisms underlying the protection from asthma, which are critical for any future development of preventive strategies.