CAREER: IDBR: Super Resolution Deep Imaging

佐治亚大学因发明新一代光学显微镜而获奖。这种显微镜将利用化学、天文学和光子学的进步，在大型生物样品中实现前所未有的分辨率。通过结合一种新的显微镜方法，光片显微镜，以及用于观察遥远星系和系外行星的技术，我们的仪器将在成像厚样品时最大限度地减少散射和像差。该仪器将光化学的最新进展与平板电视内的先进显示器相结合，将控制荧光，使其达到低于衍射极限的分辨率。这将使我们能够研究斑马鱼、果蝇、蛔虫和其他对现代生物学研究至关重要的生物的亚细胞细节。将受益于该仪器的研究实例包括斑马鱼癫痫发作的研究、果蝇的神经元放电、小鼠胚胎的眼睛发育以及发育中的秀丽隐杆线虫的细胞分裂和分化。一旦开发完成，该仪器的设计将通过一个开源模型提供给研究人员，以加速其采用。设计、软件和使用说明将在互联网上公布。最终，我们计划提供一个商业产品。通过推进生物科学和改进对作为人类疾病重要模型的生物体的研究，该项目有可能改善人类健康。该仪器创建整个多细胞生物的高分辨率荧光图像的能力将在发育生物学和其他研究整个生物细胞行为的生物研究领域发生变革。该项目将通过开发这种新型仪器，以及通过生物学家、化学家和工程师讲授的生物成像课程，培养下一代科学家进行跨学科研究。来自不同学科的学生将被介绍到生物成像的各个方面，从样品的制备和染色到显微镜的构建。该奖项是由两个项目联合颁发的，(1)生物基础设施部（生物科学理事会）的生物研究仪器开发，(2)化学、生物工程、环境和运输系统部（工程理事会）的生物光子学。

An award is made to the University of Georgia to create a next generation optical microscope. This microscope will take advantage of advances in chemistry, astronomy and photonics to achieve unprecedented resolution throughout large biological samples. By combining a new microscopy approach, light sheet microscopy, with technology used to look at distant galaxies and extrasolar planets, our instrument will minimize scattering and aberrations when imaging into thick samples. Combining recent advances in photochemistry with the advanced display inside a flat screen television, the instrument will control the fluorescence to achieve resolutions below the diffraction limit. This will allow subcellular details to be studied throughout zebrafish, fruit flies, roundworms and other organisms essential to modern biology research. Examples of research that will benefit from this instrument include the study of seizures in zebrafish, neuron firing in fruit flies, eye development in mouse embryos, and cell division and differentiation in developing C. elegans roundworms. Once developed, the instrument design will be made available to researchers through an open-source model to speed its adoption. The design, software and instructions will be made available on the internet. Ultimately, we plan to offer a commercial product.By advancing biological science and improving the study of organisms that are important models for human disease, this project has the potential to improve human health. The ability of this instrument to create high-resolution fluorescence images of entire multicellular organisms will be transformative in developmental biology and other areas of biological research in which the behavior of cells throughout organisms is studied. This project will train the next generation of scientists in interdisciplinary research through the development of this novel instrument, as well as through a course in bioimaging taught by biologists, chemists and engineers. Students from different disciplines will be introduced to all aspects of bioimaging from preparation and staining of samples to construction of a microscope.This award is being made jointly by two Programs- (1) Instrument Development for Biological Research, in the Division of Biological Infrastructure (Biological Sciences Directorate), and (2) Biophotonics, in the Division of Chemical, Bioengineering, Environmental and Transport Systems (Engineering Directorate).