Collaborative Research: IDBR Type A: QSTORM-AO - Wavefront-shaping light-sheet microscopy with photoswitchable quantum dots for superresolution imaging in thick tissue

佐治亚大学被授予开发一种用于生物研究的新型超分辨率显微镜。对于学生和职业生涯早期的研究人员来说，这种在生物、物理、光学和化学工程交叉领域的密切合作将提供宝贵的培训经验，并将随着跨学科科学交流技能的专业发展而丰富。所有团队成员将为开发一个在线团队博客、实验室笔记本和公共网站做出贡献，该在线团队博客、实验室笔记本和公共网站将在波士顿科学博物馆策划的更广泛的历史、科学和视觉背景下捕捉研究进展。科学博物馆团队还将通过现场演示、演示、播客和新星风格的短片向成千上万的科学博物馆参观者、学生和更广泛的社区传播这项工作，这些短片旨在更清晰、更深入地了解生命的奥秘。这部电影将由多位获奖的Nova制片人执导。该仪器将结合使用高分辨率空间光调制器的全息成像和纠正厚厚生物组织中光散射引起的失真的新技术，以在对现代生物学研究重要的生物(如果蝇和斑马鱼)内实现分子尺度的分辨率。在俄亥俄州立大学，半导体纳米技术将与DNA折纸技术相结合，创造出亮度极高、可切换的光发射器，这些光发射器可以单独瞄准在生物系统中工作的选定分子。这种高精度技术的新组合将为研究人员提供一个前所未有的视角，了解重要的亚细胞生物学过程，例如完整生物体中的神经元信号，可能有助于在分子和系统生物学、生物工程、医学和人类健康领域取得突破。

An award is made to the University of Georgia to develop a novel super-resolution microscope for biological research. For students and early-career researchers, this close collaboration at the intersection of biology, physics, optics, and chemical engineering will provide an invaluable training experience, and it will be enriched with professional development in interdisciplinary science communication skills. All team members will contribute to the development of an online team blog, lab notebook, and public website that will capture the progress of the research within a broader historical, scientific, and visual context, curated by the Boston Museum of Science. The Museum of Science team will also disseminate this work to thousands of science museum visitors, students, and the broader community with live presentations, demonstrations, podcasts, and a short NOVA-style film on the quest to see more clearly and deeply into the mysteries of life. The film will be directed by a multiple award-winning NOVA producer.This instrument will combine holographic imaging using high resolution spatial light modulators and new techniques for correcting distortion induced by light scattering in thick biological tissue to achieve molecular-scale resolution inside organisms important for modern biological research, such as fruit flies and zebrafish. At The Ohio State University, semiconductor nanotechnology will be combined with DNA origami to create intensely bright and switchable light emitters, individually targetable to selected molecules operating within biological systems. This novel combination of high-precision technologies will provide investigators an unprecedented view of important subcellular biological processes, such as neuronal signaling in intact organisms, potentially contributing to breakthroughs in areas of molecular and systems biology, biological engineering, medicine, and human health.