I-Corps: Lattice Automation High Throughput DNA Cloning Solutions

研究人员正在进一步开发通过合成生物学高通量克隆DNA的解决方案。该项目在数据管理、设计约束规范、DNA组装设计和实验协议规划方面进行了扩展。这些技术中的每一种都提出了算法上的挑战以及经验上的实验室挑战。用自动化的高吞吐量技术取代许多手动流程需要跨学科的设计团队、高度协调的计算/实验工作以及快速的设计-构建-测试周期来验证方法。这项工作可能会回答与人工干预最少的成分DNA设计的预测工程有关的问题。这项工作的结果可能会影响合成生物学设计的构思、设计和物理创建的方式。该团队的技术将有助于快速创造新的遗传物质，以及可供整个生物界使用的广泛适用的设计策略。这项工作有可能催生一种新兴的生物设计自动化(BDA)努力，这种努力可能涉及分子生物学的许多领域，并涉及广泛的应用(能源、材料、药物发现)。BDA的早期工作还可能创建有价值的DNA组装和数据交换标准，这将有助于统一整个领域。

Researchers are further developing solutions for high throughput cloning of DNA through synthetic biology. The project expands on data management, design constraint specification, DNA assembly design, and experimental protocol planning. These techniques each present algorithmic challenges as well as empirical laboratory challenges. The replacement of many manual processes with automated, high throughput techniques requires an interdisciplinary design team, a highly coordinated computational/experimental effort, and a rapid design-build-test cycle to validate the approach. This work may answer questions related to the predictive engineering of compositional DNA designs with minimal manual human intervention.The results of this effort may impact the way that synthetic biology designs are conceived, designed, and physically created. The team's techniques will contribute to the rapid creation of new genetic material as well as broadly applicable design strategies that can be made available to the entire biological community. This work has the potential to seed an emerging bio-design automation (BDA) effort that may touch many areas of molecular biology and engage a wide variety of applications (energy, materials, drug discovery). Early work in BDA may also create valuable DNA assembly and data exchange standards which will help to unify the entire field.